Papers by Keyword: Macroporous Resins

Abstract: The adsorption and desorption properties of Astragalus Polysaccharide (APS) (70% content) on Macroporous resins including X-5, AB-8 and D101 have been compared. APS solutions of different concentrations were tested to find a suitable sample concentration on the macroporous resins. According to our test results, AB-8 performs better than other resins. The adsorption capacity and desorption rate are 41.73mg/ml and 83.74%, higher adsorption speed for APS. Column chromatograph with AB-8 resin was used for the purification test, the sample concentration of APS is 20mg/ml, the purity of APS can reach 94.68%.

Abstract: The purification effects of six macroporous adsorption resins on total anthocyanins of hulless barley were compared. The results showed that XAD7HP was the best resin for purification of hulless barley anthocyanins due to its excellent adsorption and desorption capability. The optimal technological parameters were: the pH of sample solution was 3.0, the concentration of sample solution was 21.6 mg/L, the adsorption flow velocity was 1.0 mL/min, the eluting velocity is 1.0 mL/min, and the eluent was about 6.6 bed volumes of 80% ethanol. Reutilization test showed that the XAD7HP resin could be used repeatedly with no significant change of adsorption rate (P>0.05). After purified with XAD7HP, the color value of hulless barley anthocyanins was 41.1, increasing 9.1 times than that of raw extraction.

Abstract: Five different macroreticular resins (S-8, HPD750, AB-8, HPD100 and D101) were evaluated for the adsorption properties of the proanthocyanidins extracted from Rhodiola rose. The results showed that AB-8 displayed the optimal adsorption and desorption properties. The adsorption of AB-8 for proanthocyanidins followed Langmuir equation and belonged monolayer adsorption. The optimal purification conditions were as follows: the sample concentration of proanthocyanidins was 4mg/mL, sample flow rate was 1.5BV/h, proanthocyanidins could be entirely eluted with 3 bed volumes of 50% ethanol. Based on the experiment, the purity of proanthocyanidins product is 64%.

Abstract: The adsorption equilibrium and thermodynamics of pigment extracted from Fenugreek after degumming on macroporous resins was investigated under differentinitial concentrations. The suitability of the Freundlich and Langmuir adsorption models to the equilibrium data was investigated. The model fitness was determined by R² . Thermodynamic parameters were calculated by the Van’t Hof equation.The results showed that Freundlich model gave a better fit of adsorption isotherms than Langmuir models. The positive value of enthalpy(∆H) indicated that the adsorption was endothermic, the negative value of Gibbs free energy (∆G) showed the spontaneous and favoured nature of adsorption, and the entropy(∆S) was positive.The resins LS-46 showed an effective adsorbtion for Fenugreek yellow pigment.

Abstract: Study on Macroporous resin purified glutathione of corn germ , determine the best conditions for separation and purification as follows: glutathione and resin volume ratio 4:1, flow rate 1 mL / min ,adsorption time 50 min, elution and resin volume ratio of 4:1, analysis time 120 min, recovery rate of glutathione78.95 %.

Abstract: The optimal conditions of immobilization were obtained: the optimization of crosslinking agent concentration 10%, crosslinking time 2 hours、crosslinking temperature 40°C, pH 6.0, enzyme dilute multiples 500, PEB ion concentration 2.0M, immobilization time 6h and immobilization temperature 30°C, respectively.

Abstract: Macroporous exchange resins have been applied as supports for enzyme immobilization. The supporters were activated by cross-linking with glutaraldehyde, then Laccase was immobilized. Parameters of the immobilization process were optimized. The results of repeated batch showed the optimal immobilization conditions (10% of glutaraldehyde, pH=3, temperature=45 °C). In addition, the biocatalyst also showed good operational stability, maintaining 45% of its initial activity after 10 cycles of oxidation of ABTS. The immobilized laccase had potential for application in the continuous decolorization of textile effluents.