Papers by Keyword: Marrow Stromal Cells

Abstract: This study aims to investigate the effect of culturing conditions (static and flow perfusion) on the proliferation and osteogenic differentiation of rat bone marrow (RBM) stromal cells seeded on two starch based three-dimensional scaffolds exhibiting distinct porous structures. For this purpose, it was selected: i) a scaffold based on SEVA-C (a blend of starch with ethylene vinyl alcohol) obtained by extrusion with a blowing agent and ii) a scaffold based on SPCL (a blend of starch with polycaprolactone) obtained by a fiber bonding process. The obtained results suggest that flow perfusion culture enhances the osteogenic differentiation of RBM cells and improves their distribution in 3-D starch-based scaffolds, by improving nutrients delivery in the interior of the scaffolds and simultaneously by stimulating the seeded cells by exposing them to fluid shear forces. They also indicate that scaffold architecture and pore interconnectivity affect the homogeneity of the formed tissue.

Abstract: A previous study demonstrated that the incorporation of bioactive glass (BG) into poly (lactic-co-glycolic acid) (PLGA) can promote the osteoblastic differentiation of marrow stromal cells (MSC) on PLGA by forming a calcium phosphate rich layer on its surface. To further understand the mechanisms underlying the osteogenic effect of PLGA-BG composite scaffolds, we tested whether solution-mediated factors derived from composite scaffolds/hybrids can promote osteogenesis of marrow stromal cells. The dissolution product from PLGA-30%BG scaffold stimulated osteogenesis of MSC, as was confirmed by increased mRNA expression of osteoblastic markers such as osteocalcin (OCN), alkaline phosphatase (ALP), and bone sialoprotein (BSP). The three-dimensional structure of the scaffolds may contribute to the production of cell derived factors which promoted distant MSC differentiation. Thus PLGA-BG composites demonstrates significant potential as a bone replacement material.