Papers by Keyword: Metabolite

Abstract: We previously analyzed the dynamic flux distribution and predicted glucose, biomass concentrations of metabolic networks of E. coli by using the penalty function methods. But the consequences were not as well as we expected. In order to improve the predicated accuracy of the metabolite concentrations, instead of using Runge-Kutta algorithm, we apply Adams methods which belong to the multi-step ones in the process that solves the dynamic model of metabolic network of E. coli and obtain better simulation results on the metabolic concentrations.

Abstract: In this paper, we investigate the metabolic capabilities of two kinds cells belong to enterbacteria. Firstly we develop the mathematical models for Escherichia coli and Buchnera aphidicola Cc based on Flux balance analysis methods. Then we study their capacity of producing the important metabolite Ethanol. Finally, the results show that if the metabolic pathway is more complicated, then more the terminal metabolite-AcCoA is produced.

Abstract: The metabolies characterization of mold producing esterifying synthetase of different culture conditions were studied. A mold strain producing esterifying enzyme was isolated from Luzhou-flavor DaQu and it was identified by Biolog Microbes automated identification System. Culture conditions such as culture temperature, fermentation time and carbon source were changed. The fermentatiom broth was extracted by ethanol and analyzed by GC-MS. The results showed that the strain was Aspergillus flavus. When the culture temperature increased, the molecular weight of its metabolites increased, the synthesis of high alcohol increased too, and their carbon chain lengthened gradually. When incubation time was prolonged, the varying law of the synthesis of its metabolites was alcohols-acid-ester, the metabolites with longer carbon chain produced earlier than the metabolites with shorter carbon chain. When the carbon source were starch, sucrose and bran, n-butyl alcohol and isoamyl alcohol were the common metabolites. When the carbon source was sucrose, most of the metabolites were alcohol and acid with shorter carbon chain. When the carbon source was starch, diethyl succinate was produced. When the carbon source was bran, the metabolites were less.