Papers by Keyword: Mice

Abstract: The hydroxyapatite (HA) is a biocompatible and bioactive biomaterial used as bone substitute, however, the high crystallinity of HA and consequently its low solubility may be a limitation for its clinical use. In order to improve the biosorption of HA, the partial substitutions in the chemical structure and doping with small amounts of impurities have been study. The objective of this study was to evaluate the biocompatibility of 3% Zinc-containing nanostructured carbonated hydroxyapatite (ZncHA) compared with the carbonated hydroxyapatite (cHA), both synthesized at 37°C and non-sintered, using as control the stoichiometric HA microspheres in subcutaneous of mice. The X-ray Diffraction (XRD) and Vibrational Spectroscopy in Infra Red Fourier Transform (FTIR) were used to characterize the biomaterials. In vivo test was performed in BALB/c mice by implanting of HA, cHA and ZncHA spheres in the subcutaneous tissue for 1, and 9 weeks (n=5). The negative control consisted in incision without material implantation (Sham group). The samples were histological processed to descriptive analysis of biological effect. The microscopic analysis showed a similar granulation reaction between groups at the first experimental period. In 9 weeks there was a time dependent biosorption of cHA compared with other groups. In conclusion, the biomaterials tested were biocompatible and cHA group showed a significant biosorption in comparison with HA and ZncHA groups. The doping of zinc did not influence the biocompatibility of biomaterial, however, change the biosorption response

Abstract: Bone tissue is a composite material that has hydroxyapatite (HA) as its main inorganic phase component. The biological apatites have low crystallinity and contain cationic and anionic substitutions in their structure, which differ from the available synthetic ceramics. The purpose of this study is to evaluate the biocompatibility of nanostructured carbonated hydroxyapatite microspheres containing 5 wt% strontium (SrcHA) compared with the biocompatibility of carbonated hydroxyapatite (cHA), both synthesized at 37°C and non-sintered, used to control stoichiometric HA microspheres in subcutaneous tissue of mice. The biomaterials (BM) were characterized using X-ray Diffraction (XRD), Vibrational Spectroscopy in an Infrared Fourier Transform (VSIRFT) and Scanning Electron Microscopy (SEM). Forty five balb-C mice were randomly divided into four groups of 15 animals each: SrcHA, cHA, HA, and without material implantation (Sham group). All samples were histologically processed for descriptive evaluation of the biological effect. At each experimental period (1, 3 and 9 weeks), there was a higher biosorption of the tested biomaterials observed in contrast with the HA. The cHA group was the only group completely phagocytosed by macrophages and giant cells after 9 weeks. All biomaterials proved to be biocompatible, and the cHA and SrcHA 3% groups exhibited a faster bioabsorption in comparison with the control group. The doping of strontium did not cause a greater biological response after the 3 experimental periods.

Abstract: Solid polymeric track detectors (SPTDs), due to their insensitivity to low LET radiations and integrating nature of signal registration, are found to be effective and convenient for neutron measurements, particularly in pulsed and mixed radiation fields such as in particle accelerator environments. This paper in addition to reviewing some of the existing methods of neutron spectrometry and dosimetry, explores new approaches on the use of SPTDs for neutron measurements, elaborates on the extended energy response and rapid etching techniques of SPTDs along with some new results. Microwave induced chemical etching (MICE) technique, recently introduced for rapid and efficient processing of track detectors is discussed in the paper.Contents of Paper

Abstract: The present study aimed to determine the effects of polysaccharides from Gynostemma Pentaphyllum (PGP) supplementation on exhaustive exercise-induced fatigue in mice. The mice were divided into four experimental groups at random, i.e. control, low-dose PGP, medium-dose PGP, and high-dose PGP treatment groups. An oral administration of distilled water was given to the mice in the control group, and different doses of PGP (100, 200 and 400 mg/kg body weight/day) were given to the mice in the treatment groups. After 28 days, the weight-loaded swimming test was performed, along with the determination of blood biochemical parameters, liver glycogen and muscle glycogen contents. The results indicated that PGP supplementation had anti-fatigue effects, which could make mice swim for a longer time to exhaustion, with the blood lactic acid, serum urea nitrogen, serum triglycerides, and serum creatine kinase contents decreased, and the liver glycogen and muscle glycogen contents increased.

Abstract: The experiment was conducted to evaluate kindey selenium status, glutathione peroxidase (GPx) activity and GPx-1 expression in mice fed with nanoselenium. Sixty KM mice, female and male in half, were randomly divided into control, sodium selenite and nanoselenium groups. 0.5 milliliter of water, sodium selenite (2 μg Se/mL) and nanoselenium (2 μg Se/mL) were respectively supplemented to the three groups in oral (ig) every day. Whole experiment lasted for 28 days. Kindey selenium contents, GPx activities and GPx-1 mRNA expression were analyzed at experiment trrmination. The results showed that kindey selenium contents and GPx activities in nanoselenium group and sodium selenite group were very significantly higher than those in control group (P<0.01); kindey GPx activities in nanoselenium group were significantly higher than that in sodium selenite group (P<0.05). Kindey mRNA expression of GPx-1 was approx 166% higher in nanoselenium group and approx 157% higher in sodium selenite group than that in control group. Kindey mRNA expression of GPx-1 was approx 3.50 % higher in nanoselenium group than that in sodium selenite group. The results indicated that nanoselenium supplementation could significantly enhance kindey selenium contents, GPx activities and GPx-1 mRNA expression in mice, nanoselenium was more available than sodium selenite in increasing kindey selenium contents, GPx activities and GPx-1 mRNA expression in mice.

Abstract: The present study was carried out to investigate anti-hypoxia and anti-fatigue activities of flavonoids from bark of Eucommia ulmoides (FEU) in mice. The animal were divided into four groups: control (C) group, low-dose FEU treated (LF) group, intermediate dose FEU treated (IF) group and high-dose FEU treated (HF) group. The treated groups received FEU (5, 15, 45mg/kg), while the control group received saline solution for 28 days. After 28 days, anti-hypoxia activity of FEU was assessed by the normobarie hypoxia test and anti-fatigue activity of FEU was assessed by the forced swimming test. The data showed that FEU could prolong survival time of oxygen deprivation and exhaustive swimming time by reducing BLA and BUN levels and increasing liver glycogen and muscle glycogen contents. Therefore, FEU had anti-hypoxia and anti-fatigue activities.

Abstract: Black soybean peptides (BSPs) with potent antioxidant activity were purified from black soybean protein hydrolysates (BSH) by using the ultrafiltration (UF) and macroporous adsorption resin (MAR), and in vivo antioxidant activity of the fraction BSP-DA-c was evaluated in aging mice induced by D-galactose. The results showed that orally administration of BSP-DA-c fraction at the dose of 500 and 1000 mg/kg per day could remarkably increase (P<0.05) the activity of SOD and GSH-Px in liver and the activity of GSH-Px in serum, the contents of MDA in serum and liver were reduced significantly (P<0.05), inferred that BSP-DA-c had strong antioxidant activity in mice.

Abstract: In the present study, we investigated the anti-fatigue activity in male Kunming mice with Soy isoflavones using a forced swimming test. Mice were divided into four groups (three Soy isoflavones administered groups and a control group). The control group was gavaged with distilled water and Soy isoflavones administered groups were gavaged with Soy isoflavones (10, 20 and 40mg/10g body weight /d). After four weeks, a forced swimming test was performed and the biochemical parameters related to fatigue were examined. The results suggested that Soy isoflavones could extend the swimming time to exhaustion of the mice. This indicated that Soy isoflavones had anti-fatigue activity and could elevate the exercise tolerance.

Abstract: The objective of this study was to investigate the protective role of polysaccharide from Gynostemma pentaphyllum Makino (PGP) supplementation against exhaustive swimming exercise-induced oxidative stress. A total of 48 mice were randomly divided into four groups: control, low-dose, medium-dose, and high-dose PGP supplementation groups. The control group received distilled water and the supplementation groups received different doses of PGP (50, 100 and 200 mg/kg body weight) by gavage once a day for 28 consecutive days. After 28 days, the mice performed an exhaustive swimming exercise, and some biochemical parameters related to oxidative stress, including superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and malondialdehyde (MDA), were measured. The results showed that PGP supplementation could increase SOD, GPx and CAT contents, as well as decrease MDA contents in the liver and skeletal muscle of mice, which suggests that PGP supplementation has a protective role against exhaustive swimming exercise-induced oxidative stress.

Abstract: The present study was designed to determine the anti-hypoxia and anti-fatigue effects of Ganoderma lucidum polysaccharides (Gl-PS). The anti-hypoxia effects were evaluated by survival times of mice under anoxic condition of ordinary pressure. The anti-fatigue effects were evaluated by exhaustive swimming times of mice, and some biochemical parameters related to fatigue were measured. The results showed that Gl-PS could prolong survival and exhaustive swimming times, decrease blood lactic acid (BLA) and blood urea nitrogen (BUN) contents, and increase the liver and muscle glycogen contents of mice. Gl-PS had anti-hypoxia and anti-fatigue effects.