Papers by Keyword: Parietal

Abstract: Some microscopy studies on the mineral phase of bone have been carried out either utilizing Optical or Scanning Electron Microscopy (SEM), generally around 1000x magnification, to observe the interaction between cells and bone mineral. Other studies have been utilizing Transmission Electron Microscopy (TEM), generally around 1000000x magnification, to observe the fundamental particles that form bone mineral. The literature lacks works that utilize Scanning Electron Microscopy around 10000x magnification, particularly suitable for the study of structures composed by the aggregation of fundamental particles. The objective of this work was to study the morphologic differences between the mineral phase of femur and parietal bone of Wistar rats. Observation of the micrographs showed that the microscopic morphology of the mineral phase of femur was very similar to that of the parietal bone. It was also possible to notice that the morphology of the mineral phases varied with age, yet this variation was practically the same for the material obtained from the two types of bones.

Abstract: In this work, some modifications that occur in crystallites that compose the mineral phase of bone throughout the lives of animals were studied by X-ray diffractometry. The Debye-Scherrer equation was applied to the diffractograms, allowing the determination of the mean crystallite size and the changes in the mean crystallite size with relation to the diffraction planes 002 and 310. Likewise, the intensity of peaks in the diffractograms corresponding to the same diffraction planes was correlated with the number of crystallographic planes or of unit cells present in the crystallites.

Abstract: In this work, Scanning Electron Microscopy (SEM) has been used to determine the size and morphology of bone mineral crystals obtained from hydrazine-deproteinated parietal bone and femur of Wistar rats aged 15 days, 1 month and 1 year. Apart from the Scanning Electron Microscopy study, crystal size was also determined by X-ray diffractometry, using the Debye- Scherrer equation. Analyzing the results obtained and those reported in the literature for isolated crystals, it is possible to evaluate the influence of age and type of bone on the nanostructure of bone mineral and also propose the existence of a fundamental morphological unit that repeats itself in bone mineral formation.