Papers by Keyword: Phenotype

Abstract: The red color of red rice pericarp and aleurone is regulated by proanthocyanidin protein. Proanthocyanidin protein was encoded by partial sequence of Rc gene. The mutation from DNA sequence would give divers phenotype in pericarp and aleurone. This study used four sample of local red rice (Keli Rejo, Sumber Jaya, Cahya Tani, Sirah Pulau Padang) from South Sumatra as genetic sources and specific primers (RC12) were used to encode Proanthocyanidin protein on local red rice. This research was conducted at the Genetic and Biotechnology Laboratory of Biology Department, Faculty of Mathematics and Natural Sciences, Sriwijaya University. The primary objectives of this study were to identify the correlations between the mutations from DNA sequences which encoded proanthocyanidin protein in local red rice from South Sumatra and the fenotip of pericarp and aleurone. The stapes was DNA isolation, amplification, electrophoresis, sequencing process using First Base Malaysia and sequences analysis using Clustal W to align the DNA sequence. Sequencing result showed all of the samples had high variability (less conserved region) where the alignment process showed deletion mutation and DNA substitution mutation along the sequences of the samples. The highest mutation occurred at local red rice from Cahya Tani and the lowest mutations take place at local red rice from Keli Rejo. The samples have the same phenotype of pericarp, meanwhile different at aleurone color. The sample from Sumber Jaya had the reddest aleurone color than other sample and this sample also showed the highest deletion mutation.

Abstract: The Classical Least Square (CLS) fitting method was used to analyze the Raman spectra of living cells with the aim of identification of new phenotype-specific spectral markers for osteoblasts. The following chemicals were used for the CLS model: DNA, RNA, serum albumin, chymotrypsin and phosphatidyl choline. In this study we analyzed primary mature osteoblasts as well as two other cell types used as potential sources of osteoblasts: embryonic stem cells and fetal bone cells. The results obtained suggest that the Raman spectra of the cell types can be well approximated with a linear combination of the Raman spectra of the biopolymers used in the CLS model. The relative concentrations of the CLS components varied significantly between cell types, indicating that this analytical method could be used for phenotypic identification of osteoblasts.

Abstract: We present a new bio-photonic method based on Raman spectroscopy able to characterize living cells in in-vitro cultures. The main advantages of this technology are: no labels or other contrast enhancers are required; provides real-time analysis; cells can be maintained in physiological conditions during the measurements; no cell-damage is induced during the measurements; it is rich in information about the biochemical composition of the cell. The results show that this spectroscopic method can be used to study the most important cellular functions involved in cell-biomaterial interactions, such as cell death, differentiation, de-differentiation and mineralization. The method offers the potential for studying cell-bioceramics interaction and reduce the need of animal testing until the final steps of proving efficacy prior to clinical trials.