Papers by Keyword: Poloxamer

Abstract: The purpose of this study was to develop the formulation of chitosan-ethylenediaminetetraacetic acid (EDTA)/poloxamer containing GM extract gel. The GM extract with a concentration of 0.5% wt was incorporated into a gel formulation. The physical appearance, pH, viscosity and percentage label amount of GM extract gel were performed. The in vitro antioxidant activity of gel were evaluated using (2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The antibacterial activity against Staphylococcus aureus was evaluated by the zone of inhibition method. The mucoadhesive property was investigated using viscosity technique. The results illustrate that the chitosan-EDTA/poloxamer containing GM extract gel had a yellow colour of GM extract. The pH of a gel was in the range of 4.47 – 6.87. The percentage label amount of gel was in the range of 98.71 – 99.37% and the viscosity of gel were in the range of 14767 – 14784 mPa/s and 9607 – 9641 mPa/s. The TSol-Gel temperature was 35 oC. The antioxidant activities (IC50) which evaluated by DPPH method of all gel was in the range of 13.20 – 13.57 mg/ml. The zone of inhibition of gel against S.aureus was in the range of 8.17 – 10.52 mm. The chitosan-EDTA may improve the mucoadhesive property of gel. In conclusion, the chitosan-EDTA/poloxamer containing GM extract gel may have the potential for pharmaceutical and wound healing application.

Abstract: Normal UV-spectrophotoscopy [D0] cannot determine the combined drug in the formulation due to overlapping of the absorbance of each compound hence the derivative UV-spectrophotoscopy is used to determine the combined drug simultaneously. For determination of both hydrochlorothiazide [HCT] and propranolol hydrochloride [Pro] in powder mixture and matrix tablet, the first order derivative UV-spectrophotoscopy [D1] was employed in this study. This method showed good accuracy and precision for simultaneous determination of both drugs. Recovery was 106.59% and 97.11% for Pro and HCT, respectively. Reproducibility of both drugs was found to be less than 2.5% RSD. Repeatability was less than 2.0% of both drugs. Limit of detection (LOD) was 0.10 and 0.49 μg/ml, respectively. Limit of quantification (LOQ) was 0.31 and 1.48 μg/ml, respectively. The drug dissolution was conducted either powder mix or matrix tablet prepared by molding technique. Both drugs in powder mixture showed faster release than that from the matrix tablets. As the results, the first derivative UV-spectrophotoscopy could separate the overlapped spectra of HCT and Pro either in powder mix or in matrix tablet hence it could be used to determine the mixture of these two drugs in dosage forms.

Abstract: The ideal dermal matrix should be able to provide the right physical and biological environment to ensure homogenous cell and extracellular matrix (ECM) distribution, as well as the biocompatible interactions with tissue. Chitosan (CS)/poloxamer semi-interpenetrating polymer networks (SIPNs) was prepared by crosslinking of poloxamer macromer in the presence of CS in order to improve its mechanical property required in skin tissue engineering application. Furthermore, we also prepared CS/poloxamer SIPNs with ShebaTM, human acellular dermis, to overcome a low biocompatibility of the CS/poloxamer SIPNs. The CS/poloxamer SIPNs/ShebaTM showed remarkably highly cell attachment and viability in the two-dimensional (2D) culture and similar cell morphology on the CS/poloxamer SIPNs/ShebaTM compared with on tissue culturing polystylene (TCPS) as a positive control. These results suggest that CS/poloxamer SIPNs containing ShebaTM have good possibility for artificial skin system application.