Papers by Keyword: SSCP

Abstract: In this study, a polydimethylsiloxane (PDMS) coating filled with low concentrations of selected carboxyl modified multi-walled carbon nanotubes (cMWCNTs) has been fabricated. The antifouling properties of cMWCNTs-filled PDMS coatings were tested and the diversity level and succession phenomenon of marine fouling microbial communities were analyzed using single strand conformation polymorphism (SSCP) method. Marine adhesion test showed that cMWCNTs-filled PDMS coating presented decent antifouling property. SSCP analysis revealed that fouling prokaryotic species on the cMWCNTs-filled PDMS coating presented high and stable diversity level while diversity and dominance level of fouling eukaryotic species were relatively low, similar to those on PDMS alone and other four antifouling coating surface without nanoparticles, suggesting that the main mechanism by which cMWCNTs-mediated surface nanostructure improves antifouling capacity may have no direct relationship with the patterns in the context of succession dynamics of prokaryotic and eukaryotic microbial communities.

Abstract: Use molecular ecology technology to analyze bacteria populations in biofilm, which has a very important significance for effective sewage treatment and improvement of nitrogen and phosphorus removal rate. This article described RFLP, SSCP, FISH etc., brought together the research results at home and abroad, broadened the idea of exploring the diversity of microorganisms, the structure and distribution, as well as the dynamic in BAF.

Abstract: Single-strand conformation polymorphism (SSCP) method for detecting of HVR1 quasi-species of HCV is necessary for hepatitis C diagnosis. In this study, we described a rapid and sensitive SSCP method to analyze HCV quasi-species using common equipment. After RT-PCR reaction, denaturing was conducted with heating and Chilling. By varying the electrophoresis temperature, voltage and total acrylamide concentration，the extent of cross-linking in the gels (1-2.5%) and the concentration in glycerol ( 0-10％), SSCP method was established. With the optimized procedure, variants representing 2-3% of the whole quasi-species could be detected. By isolating visible bands from electrophoresis gel followed by sequencing, the genetic variability of HCV can be concluded. This method has high accuracy and reproducibility, simplicity and low cost, which could be a useful tool in laboratory practice.