Papers by Keyword: Scaffold

Abstract: Bone defect is a common problem in the field of dentistry. The defect can be solved bytissue engineering. One component of tissue engineering is scaffold. Carbonate apatite is the main material used because it has an organic components similar to human bones. The carbonate apatite combined with gelatin and chitosan can be used as a scaffold for tissue engineering. The aim of thisstudy is to know the exact ratio of the carbonate apatite, chitosan-gelatine (CA:Ch-GEL) scaffold on the compressive strength and porosity size as biomaterial candidates in tissue engineering. Scaffold was synthesized from CA:Ch-GEL with different ratios of 50:50, 60:40, 70:30 and 80:20 withfreeze drying method. Fourier Transform Infared Spectroscopy (FTIR) was used CA:Ch-GEL scaffold functional group identification. Scaffold mechanical test was performed using an Autograph while a porosity test was performed using Scanning Electron Microscope. All data wereanalyzed by ANOVA followed by Tukey HSD test. Scaffold has a compressive strength ranges 4.02 - 11.35 MPa, with porous ranges 19,18 mm – 52,59 mm at 50:50, 60:40, 70:30 and 80:20 ratios. CA:Ch-GEL scaffold at all ratios can be used as biomaterials in tissue engineering

Abstract: Bone tissue engineering is an alternative approach to generate bone using biomaterials and cells. Hydroxyapatite (HA) has good biocompatibility, osteoinductivity, and osteoconductivity. However, it has limited utility due to poor mechanical properties and slow degradation rate. To improve mechanical properties and to modify degradation profile, hydroxyapatite was tethered in chitosan (CS) and carboxymethyl cellulose (CMC) complex. Gelatin was incorporated to promote cell attachment and polyvinyl alcohol (PVA) was used to improve mechanical strength of this scaffold. The physico-mechanical and biological properties of these scaffolds were investigated. Fourier transform infrared (FTIR) analysis and X-ray diffraction (XRD) showed the incorporation of hydroxyapatite in polymer matrix. The scaffolds had density, compressive strength, and Young’s modulus in the range of 0.24-0.30 g/cm³, 0.028-0.035 MPa, 0.178-0.560 MPa, respectively. The scaffolds had porosity of 69-91 percent. Higher content of PVA decreased porosity of scaffolds. Scanning electron microscope showed porous microstructure with pore size in the range of 60-183 μm. In vitro test on MC3T3-E1 preosteoblast cells showed negligible cytotoxicity of scaffolds. The data suggested that HA/CS/CMC/gelatin/PVA scaffold has potential applications in bone tissue engineering.

Abstract: The study aimed to synthesize alginate hydrogel-based composites which could be injected for medical purpose and can be cured in situ gelling after the injection process. The effect of reduced graphene oxide (r-GO) addition on Alginate /poly (vinyl alcohol) (PVA) hydrogel to physical properties were evaluated. Synthesis of hydrogel Alginate/PVA/r-GO composite was previously performed by production of r-GO using Hummer method. The composition of r-GO used in composite hydrogel was 0.4, 0.8, 1.2 and 1.6% wt. The sample was then characterized using XRD, FTIR, and analyzed perform with its curing time, injectable performance, swelling ratio, and water content.

Abstract: This work aims to review current trends in research within the field of iron-based scaffolds for orthopaedic applications. Current research is trapped in a ‘see-saw’ type problem where an increase in corrosion rate of the base metal is required to accelerate the degradation process making the resorption time compatible with the healing time. This is done via several methods including porosity control, cathodic element addition and/or patterning and alloying. In turn, this increase in corrosion rate causes the local concentration of metallic ions to increase beyond the toxicity limit for osteoblast type cells, thus negatively effecting cytocompatibility. This is most pronounced when considering the orthopaedic environment, in which static conditions provide for increased local ion concentrations, resulting in local toxicity. However, research from the medical field of Thalassemia may help solve this dilemma by providing chelation medicine for patients undergoing implantation of resorbable orthopaedic scaffolds, throughout the resorption period. Excretion of iron would then be provided mainly through bowel movement and urination.

Abstract: A simple and green method for scaffold production was introduced here. The method is based on bubbling process of PVA solution. This process is superior to other conventional techniques in the matter of controllable pore size and without using of any other organic solvents. Microstructure of the scaffold was examined by a stereo microscope. Pore size and size-distribution were determined using a scanning electron microscope. Interconnected cells with uniform pores were observed without any other impurities within the pores. Average pore size was about 220 microns which is in the range required for bone tissue engineering application.

Abstract: We review here the current research status on bioreactors for tissue engineering with cell electrical stimulation. Depending on the cell types, electrical stimulation has distinct objectives, in particular being employed both to mimic and enhance the endogenous electricity measured in the natural regeneration of living organisms as well as to mimic strain working conditions for contractible tissues (for instance muscle and cardiac tissues). Understanding the distinct parameters involved in electrical stimulation is crucial to optimize its application. The results presented in the literature and reviewed here reveal that the application of electrical stimulation can be essential for tissue engineering applications.

Abstract: The case of gingival recession has a high prevalence, about 88% of the population of the United States in one or more locations suffering from gingival recession. One of the handling cases of gingival recession is to use scaffold that includes the development of tissue and cell engineering. This study aims to determine the best composition variation scaffold of collagen-chitosan with the addition of glycerol. The process of synthesis of collagen-chitosan-glycerol scaffold using freeze dry method that can form pores on the scaffold. Characterization was also carried out on the results of the synthesis of collagen-chitosan scaffold with the addition of glycerol include the morphological characterization, tensile, cytotoxicity, swelling, degradation, and thickness. The results of morphological characterization showed pore size ranged from 26.68 - 191.7 μm with a thickness of 0.51 - 0.65 mm which was suitable for handling of gingival recession cases. The result of tensile test showed that the variation of 9: 1 has the lowest value that is 2.87 MPa where the value is close to tensile strength value for periodontal which has a value ranging from 2.75 to 5.13. The characterization of cytotoxicity shows a value that is less in line with the literature, where live cells <50%. This is because collagen and chitosan have an acidic pH so that the cells cannot reproduce. Characterization of degradation shows all the variations experienced a severe reduction process from day to day. The characterization of the swelling of all samples was equilibrated at 7 minute. Chitosan-collagen scaffold with the addition of glycerol has good potential as a scaffold candidate for gingival recession therapy based on morphological characterization (thickness and surface structure), the mechanical strength (tensile strength), degradation, and the degree of swelling.

Abstract: Additive Manufacturing technologies allow for the direct fabrication of lightweight structures with improved properties. In this context, Fused Deposition Modelling (FDM) has also been considered to design 3D multifunctional scaffolds with complex morphology, tailored biological, mechanical and mass transport properties. As an example, poly (ε-caprolactone) (PCL), surface-modified PCL and PCL-based nanocomposite scaffolds were fabricated and analysed. The effects of structural and morphological features (i.e., sequence of stacking, fiber spacing distance, pore size and geometry), surface modification and nanoparticles on the in vitro biological and mechanical performances were investigated.

Abstract: Bone displays an amazing capacity for endogenous self-remodeling. However, compromised bone healing and recovering is on the ascent because of population aging, expanding rate of bone injury and the clinical requirement for the advancement of elective choices to autologous bone unions. Current strategies, including biomolecules, cell treatments, biomaterials and diverse combinations of these, are presently created to encourage the vascularization and the engraftment of the grafts, to reproduce at last a bone tissue with similar properties and attributes of the local bone. In this review, we look through the current techniques that are right now created, utilizing biomolecules, cells and biomaterials, to initiate, coordinate and potentiate bone regeneration and healing after damage and further talk about the natural procedures related with this repair.

Abstract: We previously reported that P19.CL6 cells can be cultured in porous hydroxyapatite ceramics prepared by firing green compacts consisting of apatite fibers and spherical carbon beads (150 μm in diameter). Cells cultured for 20 days in an apatite-fiber scaffold (AFS) proliferated and differentiated into cells expressing troponin T, a cardiomyocyte-specific gene, but the expression level was insufficient to support the functional maturation of cells required for biomedical device applications. In this study, we aimed to optimize the internal AFS environment for cardiomyocytes by mixing two sizes (150-and 20-μm) of carbon beads. P19.CL6 cells were cultured in AFS materials comprising different carbon ratios in the presence of alpha-MEM with (AFS+) or without (AFS-) dimethyl sulfoxide (DMSO), and cell growth and gene expression were assessed. We found that AFS(50, 1:1 ratio) is the most suitable scaffold for the proliferation and differentiation of P19.CL6 cells and the addition of DMSO to the culture medium is necessary for differentiation into cardiomyocytes. We also assessed the culture of P19.CL6 cells in AFS in a radial-flow bioreactor for several days.