Papers by Keyword: Superoxide Dismutase (SOD)

Abstract: The effects of different concentrations of nonylphenol on Neocaridina heteropoda were studied through testing the Superoxide Dismutase (SOD) activity in the muscle of N. heteropoda under the nonylphenol stress, then explored the relationship of the time that was N. heteropoda exposed nonylphenol and the in vivo physiological indicators. Results showed the following conclusion: in a certain range, higher concentrations of nonylphenol had heavier influence on SOD activity of shrimps; shrimps were infected significantly when they were exposed to the same concentration of nonylphenol in 2 days; the influence was reduced to the shrimp with the prolonging of time.

Abstract: To determine the effects of dietary selenium (Se) supplementary on muscle superoxide dismutase (SOD) activity, oriental river prawn, Neocaridina heteropoda were exposed to sodium polyphosphate. For each concentration , the shrimps were grouped into control diet group, inorganic Se group and organic Se group. For each concentration, the order of total SOD activities were organic Se diet group > inorganic Se diet group > control diet group. With the increasing concentrations of sodium polyphosphate, the total SOD activities increased first and then decreased dramatically. This study indicated that Se supplementary in diet could enhance the resistance of shrimps against low concentrations of ambient Sodium polyphosphate.

Abstract: The superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) are important ones of antioxidant defense systems. Malonaldehyde (MDA) is formed as an end product of lipid peroxidation. Soybean peptides possess antioxidant activity. In previous study, the antioxidant activities of soybean peptides were determined in vitro. The male ICR mice were intragastrically administered by different molecular weight and dose of soybean antioxidant peptides (SAP) for 60 days. Control group was treated with saline by intragastric administration for 60 days. The SOD, GSH-Px, CAT activity and MDA level were determined in serum. The results suggested the SAP of 1-3k Da had the ability to increase significantly GSH-Px and SOD activity and decrease significantly MDA level at different dose (100 and 200 mg/kg/d) compared with control group (P < 0.05). The SAP of 3-10k Da (200 mg/kg/d) enhanced the GSH-Px activity and decreased significantly MDA level compared with the control group (P < 0.05). The SAP of 10-30k Da (200 mg/kg/d) had the lowest MDA level among all the groups. All the SAP groups and positive control group cannot increase the CAT activity.

Abstract: To determine the effects of dietary selenium (Se) supplementary on muscle superoxide dismutase (SOD) activity, oriental river prawn, Macrobrachium nipponense were exposed to three commonly used pesticides - cypermethrin. For each concentration of each pesticide, the shrimps were grouped into control diet group, inorganic Se group and organic Se group. For each concentration of each pesticide, the order of total SOD activities were organic Se diet group > inorganic Se diet group > control diet group. With the increasing concentrations of pesticides, the total SOD activities increased first and then decreased dramatically. This study indicated that Se supplementary in diet could enhance the resistance of shrimps against low concentrations of ambient contaminants.

Abstract: To determine the effects of dietary selenium (Se) supplementary on muscle superoxide dismutase (SOD) activity, oriental river prawn, Neocaridina heteropoda were exposed to commonly used pesticide-parathion. The shrimps were grouped into control diet group, inorganic Se group and organic Se group. For each concentration of pesticide, the order of total SOD activities were organic Se diet group > inorganic Se diet group > control diet group. With the increasing concentrations of pesticide, the total SOD activities increased first and then decreased dramatically. This study indicated that Se supplementary in diet could enhance the resistance of shrimps against low concentrations of ambient contaminants.

Abstract: Zebra fish was used as testing animal to be stimulated by heavy metals (Cd²⁺ and Pb²⁺) in 1/20, 1/10, 1/5 half-lethal concentrations respectively. The superoxide dismutase (SOD) and catalase (CAT) were used as biomarkers to study the response of heavy metals pollutants in water on antioxidant enzyme of zebra fish. The results indicated that the activities of SOD weakened with the time increasing in the polluted water containing Cd²⁺, but the activities of CAT increased under the low concentration, and the activities trend of CAT increased at the initial period and then decreased when the concentration of Cd²⁺ increased a certain degree. Moreover, the activities of SOD became stronger along the time increasing in the polluted water containing Pb²⁺, but the enhancing trend of SOD gradually became weaker along increased concentrations, however for the activities of CAT enhanced in the initial period and then fell with the time, and the trend of decline was more and more obvious.

Abstract: In this experiment, the oxidative damage of nano-CdSeS in mice brains was performed. 20 male Kunming mice were divided into 4 groups and 3 experimental groups were exposed to different doses of nano-CdSeS (0.1 mg/mL, 0.2 mg/mL and 0.4 mg/mL) by intravenous administration while the control used saline solution instead. Three days later, the enzymatic activity of superoxide dismutase (SOD), the content of malondialdehyde (MDA) and the damage degree of DNA were determined to assess the oxidative damage in brain tissues. Our results showed that in the experimental groups, SOD activity was inhibited and MDA content was increased as the doses rising, at the same time, tail moment and tail DNA% increased significantly when comparing with the control. And these results exhibited a certain doses-dependency relations. From results above, it demonstrated that oxidative damage of brain induced by nano-CdSeS which enter into blood–brain barrier in mice.

Abstract: In order to understand Preservation effect on Brassica napus by MSOD, different concentrations of poly-aspartic manganese (II), ferrous cysteine, copper glycine are sprayed to Brassica napus, activity changes in SOD and POD and changes of MDA content are inspected. The results showed that for Brassica napus sprayed with MSOD, it makes preservation effect higher and storage period longer.

Abstract: Phyllanthus emblica L. was abundant with Vc, SOD and other nutrients. In order to find a rapid and low loss way for its solid product, spray-drying was tested on its juice. According to orthogonal experiment, the best drying condition was selected as the concentration of 0.22g/mL, input temperature of 120°C and air velocity of 0.65m3/min. With the optimal drying, it was yielded the highest amount of powder at 57.4% and 52.6% of Vc in raw juice was maintained in the dried product. The final powder was still full of SOD and its inhibition rate to autoxidation velocity of pyrogallol was 29.6%. Color, particle size, flavor, taste and resolubility were well available to solid beverage requirment. It was indicated that the technique was efficient for the drying of P. emblica L. and the dried product was anti-oxidative to some extent.

Abstract: In the present work, a hyperthermostable superoxide dismutase (SOD) was isolated from Thermus thermophilus HB27 using a membrane-based process after being expressed in E. coli. It was found that a two-stage ultrafiltration process using a 100 kDa MWCO regenerated cellulose (RC) membrane and a 50 kDa MWCO polyethersulfone (PES) membrane could successfully employed to isolate SOD from the crude feedstock. The effects of solution pH, ionic strength and permeate flux on the transmission of proteins were quantified using parameter scanning ultrafiltration. Under optimized conditions, the purity of SOD obtained was 96% with a sepcific activity of 1412 U/mg protein and the recovery of SOD from the feedback was close to 87%. The enzyme was highly stable at 90°C and retained 63% activity after heat treatment at 100°C for 1 h.