Papers by Keyword: Survival

Abstract: effect of five materials including sucrose, lactose, skim milk, yeast, vitamin B₂ on survival rate of Lactobacillus bulgaricus during freeze-drying was studied by measured survival and the number of viable cells. Designed different concentrations of these different protective agents, respectively, freeze-drying perfectly and then counted the survival ratio of Lactobacillus bulgaricus and the number of viable cells. Results were as follows: addition of skim milk and yeast had significant effects while addition of vitamin B₂ had minimal impact on survival rate of Lactobacillus bulgaricus. The optimum concentration of single protective agent of Lactobacillus bulgaricus during freeze-drying was that 25% (W/V) sucrose, 20% (W/V) lactose, 25% (W/V) skim milk, 20% (W/V) yeast and 25% (W/V) vitamin B₂, respectively, and survival rate was 24.5%, 35.6%, 64.4%, 62.2%, and 16.3%.

Abstract: Effect of MgSO₄, NaHCO₃, KH₂PO₄, K₂HPO₄ and ascorbic acid on the survival of Bifidobacterium bifidum and the number of viable cells of lyophilized powder was studied. Results were as follows: addition of MgSO₄, NaHCO₃, KH₂PO₄, K₂HPO₄ and ascorbic acid into B. bifidum paste could significantly improve the survival rate of B. bifidum and the number of viable cells of lyophilized powder. The optimal concentrations of MgSO₄, NaHCO₃, KH₂PO₄ , K₂HPO₄ and ascorbic acid were 0.20%, 0.60%, 1.80%, 1.40% and 2.20, the survival of B. bifidum was 78.31%, 77.92%, 77.80% ,79.82% and 71.62, respectively.

Abstract: Survival and germination of overwintering oospores of two strains from Gansu,China compared with two standard from Wageningen, the Netherlands of Phytophthora infestans were determined in 2010-2011. It was found that compatible mating strains of P. infestans A1 and A2 produce oospores abundantly in paired cultures on tomato-rye agar medium.The survival rate of overwintering was ranged from19.57% to 24.59%.The germination rate of overwintering was ranged from 3.58% to 8.49%, and there were no significant differences in germination rate of oospores between alternating light at day/night and whole darkness at day/night. Furthermore, the soil extract liquid was more suitable for oospore germination than distilled water.

Abstract: Four different prebiotics including fructo-oligosaccharide isomalto-oligosaccharide, inulin and xylo-oligosaccharide were screened for their effects on survival of Bifidobacterium bifidum during freeze-drying. The additions of prebiotics (v/v) were all 4%, 8%, 12%, 16% and 20%. The results showed that prebiotics could act on Bifidobacterium Bifidum. The optimum concentration of fructo-oligosaccharides (FOS) in samples for surival of Bifidobacterium bifidum was 12% and for viable count was 20％. Addition of xylo-oligosaccharides (XOS) played a significant role in promoting cell viablity when it was in low level. The optimum concentration of isomalto-oligosaccharides (IMO) for surival of Bifidobacterium bifidum was 16% and for viable count was 12％, while inulin in samples for surival and viable cell of Bifidobacterium bifidum both was 4％.

Abstract: Abstract: The experiments were conducted by planting in the field of 2 year-old Mongolian Pine (Pinus sylvstris var. mongolica) seedlings processed with Pt mycorrhizal agents, ABT rooting powder, HRC water-absorbing agent and GRR plant development and growth regulator. The results were as follows: (1) Pt₃ and ABT had significantly increased survival rates of seedlings, which were 29.3%, 23.6% and 9.4% higher than that of controlled seedlings, respectively.; (2) Pt₃ and ABT had enhanced the growth of the fine root(<2mm), which were 34.72 and 15.18 cm longer than that of controlled seedlings; the regression analysis had showed that fine roots quantity was positively related to seedling survival rate; (3) The growth indexes of the average individuals with Pt₃, ABT and HRC were higher than those of untreated seedlings; (4) Pt₃ and ABT also effectively promoted the absorption of main nutrients such as N, P, and K in the needle of seedling.

Abstract: The Omnifit-HA femoral stem component has shown excellent results in early clinical studies. This is an independent prospective study of the outcome of a ydroxyapatite-coated femoral component implanted by one surgeon with an intermediate-term follow up. The senior author performed 103 consecutive uncemented total hip arthroplasties in 96 patients from July 1991 to December 1996. The components implanted were the Omnifit-HA femoral stem and the Omnifit PSL porous-coated acetabular shell. The mean age at the time of the index procedure was 52 years old (range, 27–78) and male:female ratio was 54:42. Three patients were deceased and four patients were lost to follow-up. The mean follow up was 10.3 years (range, 7.3–12.7 years). Clinical and radiographic evaluations were performed by an independent observer. The average preoperative and postoperative Harris Hip Scores were 55 and 92, respectively. The overall survivorship of the Omnifit-HA stem was 100% with no femoral revisions. The survivorship of the Omnifit PSL cup was 89.7% with 4 acetabular revisions for aseptic loosening and 6 polyethelene liner exchanges for osteolysis or late instability. The mean polyethylene wear rate was 0.24 mm per year. This long-term follow up shows that the use of circumferentially coated hydroxyapatite stems can protect against the migration of wear debris along the femoral stem.

Abstract: Hydroxyapatite (HA) coatings for femoral stems have been successful in short- to intermediate-term follow up. However, the outcomes of HA-coated cups have not been as successful as comparable non-HA-coated designs. This has been attributed to component design and to poor quality HA-coating processes. This is a clinical outcome study of a newer generation acetabular design with an improved plasma-sprayed HA-coat. Ninety consecutive primary total hip replacements implanted with a HA-coated metal-backed cup were prospectively followed up for 2–5 years after surgery. Mean age was 69 years, male:female ratio was 36:47; mean-weight was 77 kg. Polyethylene wear was measured on digitized images. Component position, component migration, and implant-bone interface radiolucent lines were analyzed by computerized image analysis. Hip-scores improved from preoperative mean 59(±12) to a final follow-up mean of 91(±12). One hip was revised for recurrent dislocation. No hips were revised for aseptic loosening. Mean acetabular abduction angle was 46°(±8.5) and anteversion was 18°(±8.7). Incomplete lucent lines were noted on early postoperative radiographs in 5% of the cases. With ongoing follow up radiolucent line thickness decreased by mean 13% and radiolucent line density increased by 11%. No cup had complete continuous radiolucent lines or lysis. Mean polyethylene linear and volumetric wear rates were 0.14(± 0.15) mm/year and 73(± 70) mm3/year respectively. Wear rates were higher in males (p<0.03). No other factor (age, body weight, cup abduction or anteversion angle) correlated significantly with wear rate. This study presents encouraging short-term results. The improvement in radiolucent lines suggests a beneficial effect of the HA-coat on bone ingrowth. None of the cups loosened or migrated. Wear rates were comparable to published reports of successful non-HA-coated cups. Long-term follow up is ongoing to corroborate these early findings.