Papers by Keyword: Yeast Cell

Abstract: In the present work, samples were prepared allowing yeast to grow simply on YPDA-agar medium at a particular incubation temperature using streaking procedure. Doped specimens of yeast were prepared using YPDA-agar medium in presence of ZnO and TiO₂ nanoparticles in a nutrient medium for yeast. ZnO and TiO₂ nanoparticles for delivery over yeast cell were prepared with Gum Arabica and Ethanol (CH₃CH₂OH) respectively as capping agent. Specimens were also developed by laser irradiation on the ZnO doped nanoclusters and pure yeast. FTIR spectroscopy was employed to investigate the effects of nanoclusters and laser irradiation over yeast cell under different conditions. Application of laser irradiation exhibits some positive effect on pure yeast. Effect of ZnO and TiO₂ nanocluster doping on yeast are found to be toxic over Yeast Amide in general. Laser irradiation on nanocluster doped yeast cell enhanced the toxicity of nanoclusters. The later part of this study confirms the destruction of Amides in yeast. This preliminary work is an in-vivo application of drug delivery principle using ZnO nanocluster in Gum Arabica background.

Abstract: The environmental estrogen can be measured in modified yeast cells by transcription activation of reporter genes. To establish such yeast estrogen system, the full-length human estrogen receptor (hER) cDNA and estrogen responsive elements (EREs) linked to the upstream of CYC1 promoter were cloned into the yeast expression vector via pPIC9 vector respectively to construct the response vectors named as pER-PIC9 (E-9) and pERE2-CYC-PIC9K-EGFP (EC-9-E), which were transformed into Pichia pastoris at the same time for detecting environmental estrogens based on yeast Enhanced Green Fluorescence Protein (yEGFP) as a reporter protein. In the present study, we developed a rapid yeast estrogen bioassays by creating stably transfected strains with EGFP as a measurable reporter protein.