This research is concerned with the influences of cutting speed on the fatigue properties of high speed milled Ti-6.5Al-2Zr-1Mo-1V (TA15) titanium alloy. Four different cutting speeds ranging from 50 to 200m/min were used to mill the specimens for fatigue test, and the fatigue properties of them were studied at two stress levels: 80—800MPa and 90—900MPa. The fatigue lives of the specimens milled under different cutting speeds were compared. The fracture surfaces were analyzed using scanning electron microscope (SEM), and cyclic stress-strain properties of TA15 titanium alloy were investigated with a stress-strain gauge. The results showed that increasing cutting speed can help to improve the fatigue properties of titanium alloy, especially at a relatively low cyclic stress level.