Wuhan Junshan Yangtze River Bridge is an important traffic hinge on Jing-Zhu freeway, it is very necessary to conduct the comprehensive and systemic investigation on the bridge aseismic. In this paper, the FEM model of it was established; the dynamic characteristic and nonlinear seismic responses under uniform and non-uniform excitations of it were systematic studied. The results show that: 1) the basic cycle of Wuhan Junshan Yangtze River Bridge is about 8.881s. Its first mode of vibration is longitudinal floating mode, which is favorable to the earthquake- response of structures. 2) Its former 40 rank frequency are located between 0.1~2Hz which is avail to the condition of traffic condition.3) the geometric nonlinearity has much influence on the response of this kind of bridge.4) the seismic responses are sensitive to the frequency spectra of the input earthquake wave. 5) The traveling wave excitations are unfavorable to the design of tower and the main girder when considering the three orthogonal seismic wave input. In order to get correct results, artificial seismic wave of the bridge address is necessary to the time-history analysis.