A numerical simulation procedure for studying deposition of aerosol particles in a laminar convection flow of radiating gas over a backward-facing step including the effect of thermal force is developed. In the gas flow, all of the heat transfer mechanisms consisting of conduction, convection and radiation take place simultaneously. Behavior of solid particles is studied numerically based on an Eulerian–Lagrangian method. Two dimensional Navier-Stokes and energy equations are solved using CFD techniques, while the radiating transfer equation (RTE) is solved by discrete ordinate method (DOM) for calculating radiative heat flux distribution. The objective of this research is to study the effect of Reynolds number variation and also radiation on thermophoretic deposition of particles. Numerical results show a decrease in deposition percent by increasing in Reynolds number and the radiation effect is negligible. The results are compared with the existing experimental and numerical data and good agreement is found.