This study aimed at investigating the characteristics and equilibrium of Penicillium sp. QQ biosorption bacteria Exiguobacterium sp. TL. The optimal biosorption conditions were as follows: 50 mM Fe3+, 2.50 g of strain QQ, 480.80 mg/L of strain TL, 120 min of contact time, pH 5 and 40oC. The biosorption isotherms of strain TL by strain QQ could be well described by both Langmuir and Freundlich models. The maximal biosorption capacity of strain QQ for TL was 62.62 (mg/g) at 40oC, evaluated by Langmuir isotherm. It could be concluded from the results that the strain QQ was a promising biological carrier for the biosoption of strain TL, and the establishing of fungi-bacteria biosorption system was potential for pollutant removal and field application.