The synthesis of a novel chitosan (CTS) derivative bearing β-cyclodextrin (β-CD) was studied, which was divided into two steps. The first step was the preparation of carboxymethyl-β-CD, then it was acyl chlorided. The second step was that β-CD was grafted onto the CTS skeleton by the reaction of acidamide. Its adsorption property on Pb2+ was investigated. The factors that influence on the effect were discussed, including adsorption time, pH value, and temperature. The results show that its adsorption content is superior to that of the unmodified CTS. And the adsorption content (Qmax) is 246 mg.g-1 when the temperature is 35°C, pH is between 3.0~4.0 and adsorption time is 6 h.