Comparison of Two Protein Extraction Methods for Tea Residues

Abstract:

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Alcohol-alkali method and base digestion method were investigated to extract proteins from tea residues, respectively. According to single factorial experiments, results showed that the optimal extraction technology of alcohol-alkali method were pH 12, temperature of 80 °C, ethanol concentration of 60%, liquid-solid ratio of 40, 60 min, and the protein extraction rate reached 15.0%. And the optimal extract conditions of base digestion were pH 12, temperature of 80 °C, liquid-solid ratio of 50, 80 min, which made the protein yield reached 31.5%. Furthermore, alcohol-alkali method was more beneficial to protein extraction from tea residues under lower temperature and weak alkali condition (40-60 °C, pH 8-10). While base digestion had higher extraction yield under high temperature and strong alkali condition (60-80 °C, pH 11-12).

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Periodical:

Edited by:

Honghua Tan

Pages:

933-936

DOI:

10.4028/www.scientific.net/AMM.138-139.933

Citation:

X. Chen et al., "Comparison of Two Protein Extraction Methods for Tea Residues", Applied Mechanics and Materials, Vols. 138-139, pp. 933-936, 2012

Online since:

November 2011

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$35.00

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