Biocompatabiltiy of Chitosan/Poly(L-Lactic Acid) Composite Scaffolds with Three-Dimensional Honeycomb Patterned Structure

Abstract:

Article Preview

Three dimensional (3D) scaffolds provide the necessary support for cells to attach, proliferate and differentiate, and define the overall shape of the tissue engineered transplant. In this study, 3D honeycomb patterned chitosan/poly (L-lactic acid) composite scaffolds fabricated by an easy manipulated technique with good mechanical property and cytocompatability, as demonstrated by a previous study. Here we investigated further the in vitro cytocompatibility and spine regeneration in vivo by implanting the construct into male white rabbits for 4 and 8weeks. Results showed that such a honeycomb patterned scaffolds have a good cytocompatibilty. Also, the rabbit spinal defect was perfectly restored. These findings supported that such a 3D honeycomb patterned scaffold is an ideal candidate for the tissue engineering scaffold.

Info:

Periodical:

Edited by:

Robert Zhu

Pages:

29-33

DOI:

10.4028/www.scientific.net/AMM.140.29

Citation:

M. Y. Zhao et al., "Biocompatabiltiy of Chitosan/Poly(L-Lactic Acid) Composite Scaffolds with Three-Dimensional Honeycomb Patterned Structure", Applied Mechanics and Materials, Vol. 140, pp. 29-33, 2012

Online since:

November 2011

Export:

Price:

$35.00

[1] Langer R, Vacanti JP. Tissue engineering. Science. 260 (1993) 920-926.

[2] Engler Adam J, Sen S, Lee Sweeney H, et al. Matrix elasticity directs stem cell lineage specification. Cell. 126 (2006) 677–689.

DOI: 10.1016/j.cell.2006.06.044

[3] Yukako Fukuhira, Eiichi Kitazono, Takami Hayashi, et al. Biodegradable honeycomb-patt erned film composed of poly (lactic acid) and dioleoylphosphatidylethanolamine. Biomaterials. 27 (2006) 1797-1802.

DOI: 10.1016/j.biomaterials.2005.10.019

[4] Boccafoschi F, Habermehl J, Vesentini S, et al. Biological performances of collagen-based scaffolds for vascular tissue engineering. Biomaterials. 26 (2005) 7410–7417.

DOI: 10.1016/j.biomaterials.2005.05.052

[5] Ravi Kumar M. A review of chitin and chitosan applications. React Funct Polym. 46(2000) 1–27.

[6] Couet F, Rajan N, Mantovani D. Macromolecular biomaterials for scaffold-based vascular tissue engineering. Macromol Biosci. 7 (2007) 701–718.

DOI: 10.1002/mabi.200700002

[7] Huang Y, Siewe M, Madilhally SV. Effect of spatial architecture on cellular colonization. Biotechnol Bioeng. 93 (2006)64–75.

[8] S.S. Kim, M.S. Min Sun Park, O. Jeon, et al. Biomaterials. 27 (2006)1399–1409.

[9] D.K. Kim, H.S. Kim. Structure and characteristic of chitosan Bombyx mori silk fibroin blend films. Polym-Korea. 29 (2005) 408–412.

[10] Mathew Peter, Nitya Ganesh, N. Selvamurugan, et al. Preparation and characterization of chitosan– gelatin/nanohydroxyapatite composite scaffolds for tissue engineering applications. Carbohydrate Polymers. 80 (2010) 687-694.

DOI: 10.1016/j.carbpol.2009.11.050

[11] Mingyan Zhao, Lihua Li, Xian Li, et al. Three-dimensional honeycomb-patterned chitosan /poly (L-lactic acid) scaffolds with improved mechanical and cell compatibility. Journal of biomedical materials research part A. (2011).

DOI: 10.1002/jbm.a.33132

[12] M.H. Stenzel, Aust. Formation of Regular Honeycomb-Patterned Porous Film by Self-Organization. Chem. 55 (2002) 239-243.

[13] M. Srinivasarao, D. Collings, A. Three-Dimensionally Ordered Array of Air Bubbles in a Polymer Film. Philips and S. Patel, Science, 293 (2000) 79–83.

DOI: 10.1126/science.1057887

In order to see related information, you need to Login.