Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A
We have successfully developed gold nanoparticle based immunostrip assay to detect protein-A (PA). Rabbit polyclonal antibody IGg (αPA) that has affinity to PA was conjugated to gold nanoparticles (GNPs) and the gold nanoconjugate (αPA-GNP) was used to detect protein-A by simple immunostrip assay method. ELISA experiments were used to confirm the retention of binding affinity of antibody towards protein-A after conjugation with gold nanoparticles.
A. Zambre et al., "Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A", Applied Mechanics and Materials, Vols. 229-231, pp. 260-266, 2012