Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A

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We have successfully developed gold nanoparticle based immunostrip assay to detect protein-A (PA). Rabbit polyclonal antibody IGg (αPA) that has affinity to PA was conjugated to gold nanoparticles (GNPs) and the gold nanoconjugate (αPA-GNP) was used to detect protein-A by simple immunostrip assay method. ELISA experiments were used to confirm the retention of binding affinity of antibody towards protein-A after conjugation with gold nanoparticles.

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Periodical:

Edited by:

Mohamed Othman

Pages:

260-266

Citation:

A. Zambre et al., "Gold Nanoparticle Based Immunostrip Assay Method for Detection of Protein-A", Applied Mechanics and Materials, Vols. 229-231, pp. 260-266, 2012

Online since:

November 2012

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$38.00

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