Design and Characterization of Precise Laser-Based Autofocusing Microscope with Reduced Geometrical Fluctuations


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The present study proposes a laser-based microscope system in which a precise autofocusing capability is achieved using a position feedback signal based on the distance L between the geometrical center (Xc, Yc) of the image captured by the CCD sensor and the centroid (xcentroid, ycentroid) of the image. The experimental results show that the proposed system has a positioning accuracy of 2.2 μm given a working range of ±200 μm. The autofocusing performance of the proposed system is thus better than that of a conventional centroid-based system, which typically achieves a positioning accuracy of around 5.2 μm.



Edited by:

Wen-Hsiang Hsieh




C. S. Liu et al., "Design and Characterization of Precise Laser-Based Autofocusing Microscope with Reduced Geometrical Fluctuations", Applied Mechanics and Materials, Vols. 284-287, pp. 672-676, 2013

Online since:

January 2013




[1] T. Pengo, A. Munoz-Barrutia, C. Ortiz-De-Solorzano, Halton sampling for autofocus, J. Microsc. 235 (2009) 50-58.

DOI: 10.1111/j.1365-2818.2009.03180.x

[2] H.C. Chang, T.M. Shih, N.Z. Chen, N.W. Pu, A microscope system based on bevel-axial method auto-focus, Opt. Lasers Eng. 47 (2009) 547-551.

DOI: 10.1016/j.optlaseng.2008.10.004

[3] S.J. Abdullah, M.M. Ratnam, Z. Samad, Error-based autofocus system using image feedback in a liquid-filled diaphragm lens, Opt. Eng. 48 (2009) 123602-1-123602-9.

DOI: 10.1117/1.3275450

[4] H.G. Rhee, D.I. Kim, Y.W. Lee, Realization and performance evaluation of high speed autofocusing for direct laser lithography, Rev. Sci. Instrum. 80 (2009) 073103-1-073103-5.

DOI: 10.1063/1.3176468

[5] K.H. Kim, S.Y. Lee, S. Kim, S.G. Jeong, DNA microarray scanner with a DVD pick-up head, Curr. Appl. Phys. 8 (2008) 687-691.

DOI: 10.1016/j.cap.2007.04.047

[6] S. Gossler et al., Mode-cleaning and injection optics of the gravitational-wave detector GEO600, Rev. Sci. Instrum. 74 (2003) 3787-3795.

[7] S. Avino et al., High sensitivity adaptive optics control of laser beam based on interferometric phase-front detection, Opt. Lasers Eng. 45 (2007) 468-470.

DOI: 10.1016/j.optlaseng.2005.07.013

[8] A. Weiss, A. Obotnine, A. Lasinski, U.S. Patent 7700903B2, (2010).

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