Study on Gold Nanoparticles Aggravating MEMS Cantilever Gene Detection Biosensor
In order to detect whether there are nucleic acid components interested in the testing samples, this study manuscript presents a method and a process based on MEMS technology and biosensor technology. Although MEMS micro cantilever is very sensitive for stress mass, we must aggravate the cantilever for the reason of microscopic DNA mass is very weak. In order to achieve the purpose of their molecules detection level, gold nanoparticles (GNPs) which are combined with hybridization information is taken to aggravate MEMS cantilever, and we employ Au - S chemical bond of GNPs and dithiol HS(CH2)6SH to combine and fix DNA probe, and then employ target DNA which is marked with biotin to combine GNPs by Biotin - Streptavidin combining. We use the method of layer by layer to amplify the signal again until meet the hypersensitized requirements. Last we apply multi-channel microfluidic technology to achieve high-throughput detection.
Prasad Yarlagadda and Yun-Hae Kim
Y. T. Ge et al., "Study on Gold Nanoparticles Aggravating MEMS Cantilever Gene Detection Biosensor", Applied Mechanics and Materials, Vols. 336-338, pp. 148-152, 2013