Frame is an important truck part to connect and support engine, transmission, carriage and cab etc. It is important to analyze strength, stiffness and dynamic characteristics for frame design. This paper developed static analysis of a specific agricultural truck frame under two conditions of full load bending and reverse. The corresponding suggestions of structural modification were proposed. Then the dynamic response of truck frame caused by the external incentives was studied, namely harmonic response analysis and transient dynamic analysis. The paper not only analyzed whether the truck frame met the strength requirements, but also proposed the corresponding solutions for the possible cases of fatigue fracture and stress overloaded of the frame.