Micro-Measurement of the Virus-Infected Region Using NIS-Elements System

Abstract:

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A method was successfully developed for the measurement of the virus-infected region and distribution of virus. The system consists of a in situ PCR instrument, a light microscope, and an image processing and analysis software. Through developing a in situ RT-PCR system for location of a strawberry virus, the sections were observed on a Nikon light microscope by magnifying 400×. Alcian blue precipitate was observed in palisade tissues, and it indicated that the virus was located in the tissue. The area of the virus-infected region was measured by NIS-Elements software. At last, the distribution of virus in tissue and the area of infected region could be analyzed and accounted on the basis of the micro-measured results. The average area of forty virus-infected regions was 1.5×103 µm2, in addition, the ratio of the area of healthy region to virus-infected one was 3.4. The system achieved to measure quantitatively the virus-infected tissues dispensing with the complicated transmission electron microscope (TEM).

Info:

Periodical:

Edited by:

Ran Chen

Pages:

1628-1632

DOI:

10.4028/www.scientific.net/AMM.44-47.1628

Citation:

H. Y. Yang et al., "Micro-Measurement of the Virus-Infected Region Using NIS-Elements System", Applied Mechanics and Materials, Vols. 44-47, pp. 1628-1632, 2011

Online since:

December 2010

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$35.00

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