Early Apoptosis Difference and Mechanism of Hepatic Cell Lines Treated by Extremely Low Frequency Altering Electro-Magnetic Field Combined with Magnetic Nano Fe3O4 Particles In Vitro
Extremely low frequency electro-magnetic field (ELFF) exposure could induce hepatoma Bel-7402 and SMMC-7721 cells lines into early apoptosis without some significant effects to healthy hepatic cells HL-7702 cells in low exposure dose through affecting cellular membrane ion metabolism. Magnetic nanoFe3O4 particles (MNPs) presence with low effects to all hepatic cells lines strengthened ELFF bio-effects to tumor cells inducing more amount of tumor cells into early apoptosis with low effects to HL-7702 cells. Western-blot results indicated the early apoptosis signal channel Bcl protein family of tumor cells was activated significantly more effectively than HL-7702 cells due to higher MNPs uptake ratio of tumor cells and higher cellular membrane ion exchanging current than HL-7702 cells. Patch-clamp results showed MNPs combined with ELFF treatment inducing cells into early apoptosis should be partially based on ion metabolism disturbance of cells with similar to ELFF bio-effects to cells.
X.D. Xu, Bin Li, Q.M. Lu, X.Y. Yan and J.L. Li
Z. Q. Chen et al., "Early Apoptosis Difference and Mechanism of Hepatic Cell Lines Treated by Extremely Low Frequency Altering Electro-Magnetic Field Combined with Magnetic Nano Fe3O4 Particles In Vitro", Applied Mechanics and Materials, Vols. 556-562, pp. 539-546, 2014