Extraction and SDS-PAGE Analysis of Proteins from Gualou Seeds

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Protein extraction from Gualou seeds were studied, and Kjeldahl method and Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) were used to characterize the properties of extracted proteins. The optimum extraction parameters were as follows: the extraction solution was 0.2 mol/L phosphate buffer (pH, 8.0) with 5% NaCl, and the ratio of solid to liquid was 1:10. A protein yield of 75.01% was obtained under the optimal extraction conditions. The SDS-PAGE analysis showed that the proteins extracted from Gualou seeds have apparent molecular weights varying from 10 to 30 kDa, mainly close to 30 kDa.

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Edited by:

Zhongmin Wang, Dongfang Yang, Kun Yang, Liangyu Guo and Jianming Tan

Pages:

5193-5196

Citation:

J. B. Zhao et al., "Extraction and SDS-PAGE Analysis of Proteins from Gualou Seeds", Applied Mechanics and Materials, Vols. 644-650, pp. 5193-5196, 2014

Online since:

September 2014

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$38.00

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