Extraction and SDS-PAGE Analysis of Proteins from Gualou Seeds
Protein extraction from Gualou seeds were studied, and Kjeldahl method and Sodium dodecyl sulfonate-polyacrylamide gelelectrophoresis (SDS-PAGE) were used to characterize the properties of extracted proteins. The optimum extraction parameters were as follows: the extraction solution was 0.2 mol/L phosphate buffer (pH, 8.0) with 5% NaCl, and the ratio of solid to liquid was 1:10. A protein yield of 75.01% was obtained under the optimal extraction conditions. The SDS-PAGE analysis showed that the proteins extracted from Gualou seeds have apparent molecular weights varying from 10 to 30 kDa, mainly close to 30 kDa.
Zhongmin Wang, Dongfang Yang, Kun Yang, Liangyu Guo and Jianming Tan
J. B. Zhao et al., "Extraction and SDS-PAGE Analysis of Proteins from Gualou Seeds", Applied Mechanics and Materials, Vols. 644-650, pp. 5193-5196, 2014