Application of 3D Traction Force Microscopy to Mechanotransduction of Cell Clusters
With increasing understanding of the important role mechanics plays in cell behavior, the experimental technique of traction force microscopy has grown in popularity over the past decade. While researchers have assumed that cells on a flat substrate apply tractions in only two dimensions, a finite element simulation is discussed here that demonstrates how cells apply tractions in all three dimensions. Three dimensional traction force microscopy is then used to experimentally confirm the finite element results. Finally, the implications that the traction distributions of cell clusters have on the study of inhibition of proliferation due to cell contact and scattering of cells in a cluster are discussed.
R. L. Burguete, M. Lucas, E. A. Patterson, S. Quinn
J. Notbohm et al., "Application of 3D Traction Force Microscopy to Mechanotransduction of Cell Clusters", Applied Mechanics and Materials, Vol. 70, pp. 21-27, 2011