Deep water coastline and ecological environment are natural resources that the development of port city will depend on. Driven by economy globalization, port city development is the inevitable choice to guarantee economic flow and promote sustainable development of trading. Especially in newly rising developing countries, the construction of port city and green city should be organically combined, and the ecological environment protection becomes the important work for construction of green port city. Meishan Island in Ningbo is just at the critical period for determing the long-term development orientation. Meishan Island is the only undeveloped area in Ningbo City, which has deep water coastline, land resources, ecological environment and cultural tourism resources. In early 2007, China Academy of Urban Planning and Design is appointed by Beilun District Government of Ningbo City to perform a demonstration of long-term development orientation for Meishan Island. The topic is “Investigation of Ningbo Meishan Island Development”. The development plan and ecological environment protection plan for deep water port city was investigated on the base of this project, and some references for investigation were provided on ecological environment protection plan for deep water port city. The natural geographical condition of Ningbo Meishan Island was introduced as well as its role in development of regional economy and society; performed factor analysis for ecological environment of Meishan Island. Considering the level of ecological protection plan, Meishan Island is divided into three areas by functions which are port city development zone, ecological buffer zone and ecological protection zone.