Effect of Jasminum sambac Flower Extracts on Fibroblast Cell Viability and Film Characteristics

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Jasminum sambac (J. sambac) has been used as a medicinal herb with wound healing property. This experimental research proposed to evaluate the effect of J. sambac flower extracts on fibroblast cell viability and film characteristics which maybe benefit in development of wound dressing film. The testing extract of J. sambac flowers were prepared as filtration extract (F), compression of the residue flower extract (C), combination of filtration and compression extract (FC) and ethanolic extract (E). The testing extracts were evaluated the effect on fibroblast cell viability using resazurin reduction assay. NIH 3T3 fibroblast cells were treated with variable concentration of J. sambac extracts for 24, 48 and 72 h. prior to evaluate cell viability. The results demonstrated that the F and FC extract of J. sambac flower at concentration of 31.25-500 µg/ml did not induce cytotoxicity on NIH 3T3 fibroblast cells given non-cytotoxic effect. The cell viability seemed to increase at 48 h of treatment with concentration of 31.25-250 µg/ml. Meanwhile, C and E extract induced cytotoxicity on NIH 3T3 cells by reduction of cell viability. The F and FC non-cytotoxic extracts were selected to examine ability of film formation without film-based compounds and assessed physical and mechanical characteristics of the prepared films. The experimental results revealed F and FC extracts could form film after incubation at 60°C. The better physical and mechanical characteristics of the films was presented in FC film which the prepared film revealed smooth surface and better absorption and elongation. Furthermore, gelatin was blended to FC extract for assess improvement of film properties. The results revealed that gelatin-blended FC extract film gave better mechanical characteristics of the film. Non-cytotoxicity of the FC extract of J. sambac flowers on fibroblast cells and appropriate physical and mechanical characteristics of FC film may point to the usefulness of J. sambac flower extracts in herb-based film for wound dressing.

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Edited by:

Ruangdet Wongla

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27-33

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P. Eakwaropas and N. Wisidsri, "Effect of Jasminum sambac Flower Extracts on Fibroblast Cell Viability and Film Characteristics", Applied Mechanics and Materials, Vol. 886, pp. 27-33, 2019

Online since:

January 2019

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[1] A. Mittal, S. Sardana, A. Pandey, Ethnobotanical, phytochemical, pharmacological profile of Jasminum sambac (L.) Ait., Journal of J Pharm Biomed Sci. 11 (2011) 1-7.

[2] M. Chopda, R. Mahajan, Wound Healing Plants of Jalgaon District of Maharashtra State, India, Ethnobotanical Leaflets, 13 (2009) 1-32.

[3] P. Kunhachan, C. Banchonglikitkul, T. Kajsongkram, A. Khayungarnnawee, W. Leelamanit, Chemical Composition, Toxicity and Vasodilatation Effect of the Flowers Extract of Jasminum sambac (L.) Ait. G. Duke of Tuscany,, Evid-Based Compl Alt. (2012).

DOI: https://doi.org/10.1155/2012/471312

[4] P. Shrivastav, K. George, N. Balasubramaniam, M.P. Jasper, M. Thomas, A.S. Kanagasabhapathy, Suppression of puerperal lactation using jasmine flowers (Jasminum sambac), Aust N Z J Obstet Gynaecol. 28 (1988) 68-71.

DOI: https://doi.org/10.1111/j.1479-828x.1988.tb01614.x

[5] C.C. Rath, S. Devi, S.K. Dash, R.K. Mishra, Antibacterial Potential Assessment of Jasmine Essential Oil against E-coli, Indian J Pharm Sci. 70 (2008) 238-U235.

DOI: https://doi.org/10.4103/0250-474x.41465

[6] B. Rambabu, K.S.K. Rao Patnaik, Phytochemical screening and evaluation of analgesic, anti-inflammatory activity of alcoholic extract of Jasminum sambac on albino rats, World journal of pharmacy and pharmaceutical sciences. 3 (2014) 547-555.

[7] N. Sengar, A. Joshi, S.K. Prasad, S. Hemalatha, Anti-inflammatory, analgesic and anti-pyretic activities of standardized root extract of Jasminum sambac, J Ethnopharmacol. 160 (2015) 140-148.

DOI: https://doi.org/10.1016/j.jep.2014.11.039

[8] E.A. Kamoun, E.S. Kenawy, X. Chen, A review on polymeric hydrogel membranes for wound dressing applications: PVA-based hydrogel dressings, J Adv Res. 8 (2017) 217-233.

DOI: https://doi.org/10.1016/j.jare.2017.01.005

[9] P. Ganesan, Natural and bio polymer curative films for wound dressing medical applications, Wound Medicine. 18 (2017) 33-40.

DOI: https://doi.org/10.1016/j.wndm.2017.07.002

[10] A.C. Gonzalez, T.F. Costa, Z.A. Andrade, A.R. Medrado, Wound healing - A literature review, An Bras Dermatol. 91 (2016) 614-620.

DOI: https://doi.org/10.1590/abd1806-4841.20164741

[11] I.A. Darby, B. Laverdet, F. Bonte, A. Desmouliere, Fibroblasts and myofibroblasts in wound healing, Clin Cosmet Investig Dermatol. 7 (2014) 301-311.

DOI: https://doi.org/10.2147/ccid.s50046

[12] D. Gourevich, L. Ofek, Y. Eichbaum, L. Wertheim, R. Gotlieb, Fibroblasts Adhesion to Soy Protein Films for Wound Healing: an In vitro Study, JSM Burns Trauma. 2 (2017) 1029.

[13] M. Kalaiselvi, R. Narmadha, P. Ragavendran, G. Ravikumar, D. Gomathi, D. Sophia, C. Arul Raj, C. Uma, K. Kalaivani, In vivo and in vitro antitumor activity of Jasminum sambac (Linn) Ait oleaceae flower against dalton's ascites lymphoma induced swiss albino mice, Int J Pharm Pharm Sci. 4 (2011) 144-147.

DOI: https://doi.org/10.1016/s2221-1691(11)60158-5

[14] W.B. Li, A.D. Kandhare, A.A. Mukherjee, S.L. Bodhankar, Hesperidin, a Plant Flavonoid Accelerated the Cutaneous Wound Healing in Streptozotocin-Induced Diabetic Rats: Role of Tgf-B/Smads and Ang-1/Tie2 Signaling Pathways, Excli J, 17 (2018) 399-419.

[15] K.D. Arunachalam, S. Subhashini, Preliminary phytochemical investigation and wound healing activity of Myristica andamanica leaves in Swiss albino mice, J Med Plants Res. 5 (2011) 1095-1106.

[16] R.F. Pereira, P.J. Bartolo, Traditional Therapies for Skin Wound Healing, Adv Wound Care. 5 (2016) 208-229.

DOI: https://doi.org/10.1089/wound.2013.0506

[17] R. Pereira, A. Tojeira, D.C. Vaz, A. Mendes, P. Bartolo, Preparation and Characterization of Films Based on Alginate and Aloe Vera, Int J Polym Anal Ch. 16 (2011) 449-464.

DOI: https://doi.org/10.1080/1023666x.2011.599923