Pretreatment Techniques for Arsenic Speciation Analysis in Biosamples


Article Preview

Sample pretreatment techniques for arsenic speciation analysis in biosamples are of significant importance in detection procedures. In this paper, several typical pretreatment techniques such as solvent extraction (SE), enzymatic hydrolysis (EH), pressurized liquid extraction (PLE), solid phase microextraction (SPME) and liquid phase microextraction (LPME) were summarized on the basis of 20 references. The new trend in the future was also carried out.



Edited by:

Z.S. Liu, L.P. Xu, X.D. Liang, Z.H. Wang and H.M. Zhang




J. L. Guo et al., "Pretreatment Techniques for Arsenic Speciation Analysis in Biosamples", Advanced Materials Research, Vol. 1015, pp. 359-362, 2014

Online since:

August 2014




* - Corresponding Author

[1] Gomez-Ariza, J.L., Sánchez-Rodas, D., Beltran, R. Evaluation of atomic fluorescence spectrometry as a sensitive detection technique for arsenic speciation [J]. Applied Organometallic Chemistry, 1998, 12 (6): 439–447.

[2] Lamble, K.J., Hill, S.J. Arsenic speciation in biological samples by on–line high performance liquid chromatography-microwave digestion-hydride generation-atomic absorption spectrometry [J]. Analytica Chimica Acta, 1996, 334 (3): 261–270.

[3] Wrobel, K., Caruso, J.A. Pretreatment procedures for characterization of arsenic and selenium species in complex samples utilizing coupled techniques with mass spectrometric detection [J]. Analytical and Bioanalytical Chemistry, 2005, 381 (2): 317–331.

[4] Wuilloud, R.G., Vonderheicle, A.P., Caruso, J.A., et al. Determination of iodinated phenol species at parts–per–trillion concentration levels indifferent water samples by solid-phase microextraction/offline GC–ICP–MS [J]. Journal of Analytical Atomic Spectrometry, 2003, 18 (9): 1119–1124.

[5] Sanchez, W.M., Zwicker, B., Chatt, A. Determination of As(III), As(V), MMA and DMA in drinking water by solid phase extraction and neutron activation [J]. Journal of Radioanalytical and Nuclear Chemistry, 2009, 282 (1): 133–138.


[6] Geng, W.H., Komine, R., Ohta, T., et al. Arsenic speciation in marine product samples: Comparison of extraction–HPLC method and digestion–cryogenic trap method [J]. Talanta, 2009, 79 (2): 369–375.


[7] Kirby, J., Maher, W. Measurement of water–soluble arsenic species in freeze–dried marine animal tissues by microwave-assisted extraction and HPLC–ICP–MS [J]. Journal of Analytical Atomic Spectrometry, 2002, 17 (8): 838–843.


[8] Santos, C.M., Nunes, M.A., Barbosa, I.S., et al. Evaluation of microwave and ultrasound extraction procedures for arsenic speciation in bivalve mollusks by liquid chromatography–inductively coupled plasma-mass spectrometry [J]. Spectrochimica Acta Part B, 2013, 86 (1): 108–114.

[9] Piñeiro, J.M., Rodríguez, E.A., Piñeiro, A.M., et al. Simultaneous pressurized enzymatic hydrolysis extraction and clean up for arsenic speciation in seafood samples before high performance liquid chromatography–inductively coupled plasma–mass spectrometry determination [J]. Analytica Chimica Acta, 2010, 679 (1–2): 63–73.

[10] Branch, S., Ebdon, L., O'Neill, P. Determination of arsenic species in fish by directly coupled high-performance liquid chromatography–inductively coupled plasma mass spectrometry [J]. Journal of Analytical Atomic Spectrometry, 1994, 9 (1): 33–37.

[11] Piñeiro, A.M., Piñeiro, J.M., Hermelo, P.H., et al. Application of fast ultrasound water-bath assisted enzymatic hydrolysis–High performance liquid chromatography–inductively coupled plasma-mass spectrometry procedures for arsenic speciation in seafood materials [J]. Journal of Chromatography A, 2011, 1218 (39): 6070–6980.


[12] Barrera, P.B., Piñeiro, A.M., Barrera, A.B. Sample pre-treatment methods for the trace elements determination in seafood products by atomic absorption spectrometry [J]. Talanta, 2002, 57 (5): 969–984.

[13] Fernández, M.J., Rey, G.R., Piñeiro, J.M., et al. Arsenic extraction in marine biological materials using pressurised liquid extraction [J]. Talanta, 2007, 71 (2): 515–520.


[14] Pérez, C.M., Piñeiro, J.M., Mahía, P.L., et al. Pressurized liquid extraction followed by high performance liquid chromatography coupled to hydride generation atomic fluorescence spectrometry for arsenic and selenium speciation in atmospheric particulate matter [J]. Journal of Chromatography A, 2008, 1215 (1–2): 15–20.

[15] Rodríguez, E.A., Piñeiro, J.M., Mahía, P.L., et al. Pressurized liquid extraction of organometals and its feasibility for total metal extraction [J]. TrAC Trends in Analytical Chemistry, 2006, 25 (5): 511–519.

[16] Tamer, U., Yates, B., Galal, A., et al. Electrochemically aided control of solid phase micro–extration (EASPME) using conducting polymer-coated solid substrates applicate to neutral analytes [J]. Microchimica Acta, 2003, 143 (2–3): 205–215.

[17] Szostek, B., Aldstadt, J.H. Determination of organoarsenicals in the environment by solid–phase microextraction–gas chromatography–mass spectrometry [J]. Talanta, 1998, 807 (2): 253–263.

[18] Jiang, H.M., Chen, B.B., Xia, L.B. Hollow fiber liquid phase microextraction combined with electrothermal atomic absorption spectrometry for the speciation of arsenic (III) and arsenic (V) in fresh waters and human hair extraction [J]. Analytica Chimica Acta, 2009, 634 (1): 15–21.

[19] Xia, L.B., Hu, B., Wu, Y.L. Hollow fiber–based liquid–liquid–liquid microextraction combined with high–performance liquid chromatography for the speciation of organomercury [J]. Journal of Chromatography A, 2007, 1173 (1–2): 44–51.

[20] Yu, X.P., Deng, T.L., Guo, Y.F. Arsenic species analysis in freshwater using liquid chromatography combined to hydride generation atomic fluorescence spectrometry [J]. Journal of Analytical Chemistry, 2014, 69 (1): 83–88.