Nanometer TiO2 microcapsule has potential applications in biochemistry, drug Controlled Release, and catalyst. Meanwhile, Ultraviolet (UV) light-induced bioactivity of titanium substrates is attracting more and more attention nowadays. Composite thin films formed by Nano-TiO2 microcapsule prepared by a novel and versatile technique of layer-by-layer (L-b-L) deposition using the Nanometer latex particles of Polystyrene (PS) as the template together with TiO2 films synthesized at low temperature by the hydrolysis of titanium chloride (TiCl4) precursor was successfully deposited on the different titanium substrates after the template was removed. Thus great expectation was placed on whether UV radiation can enhance the bioactivity of the titanium substrates after the composite thin films being deposited on its surface. The apatite-forming ability was evaluated after the UV-irradiated implants being soaked in simulated body fluid (SBF) within different hours by Scanning electron microscopy (SEM), XRD. The vitro results indicated that UV radiation was favored to formation of apatite on titanium substrates. Besides, the effect of different UV- irradiation time on apatite-forming was different.