Cytotoxicity of pH-Responsive Dextrin Nanogels against Human Osteosarcoma 143B Cells

Abstract:

Article Preview

To overcome major side-effects, that is, cardiotoxicity of doxorubicin (DOX), pH-responsive dextrin nanogels (DNGs) were developed for using as a smart drug carrier. DOX-loaded DNGs were fabricated by emulsion cross-linking technique using glyoxal as a cross-linking agent to form acid-sensitive, acetal bonds. The objective of this study was to investigate the cytotoxicity of DOX-loaded DNGs on the human osteosarcoma 143B cell line. The cytotoxicity assay results showed that DOX-loaded DNGs retained high cell inhibition efficiency in 143B cells in a concentration- and treatment time-dependent manner. The cytotoxicity decreased with increasing ratio of glyoxal to dextrin. Observation of 143B cells by light microscopy showed the morphological changes after treatment with DOX-loaded DNGs. These results suggested that DOX-loaded DNGs with pH-sensitive properties is promising for use as a drug delivery system for cancer therapy.

Info:

Periodical:

Edited by:

Pornsak Sriamornsak, Sontaya Limmatvapirat and Suchada Piriyaprasart

Pages:

227-230

Citation:

S. Manchun et al., "Cytotoxicity of pH-Responsive Dextrin Nanogels against Human Osteosarcoma 143B Cells", Advanced Materials Research, Vol. 1060, pp. 227-230, 2015

Online since:

December 2014

Export:

Price:

$38.00

* - Corresponding Author

[1] H.T. Ta, C.R. Dass, I. Larson, P.F.M. Choong, D.E. Dunstan, A chitosan-dipotassium orthophosphate hydrogel for the delivery of Doxorubicin in the treatment of osteosarcoma, Biomaterials. 30 (2009) 3605-3613.

DOI: https://doi.org/10.1016/j.biomaterials.2009.03.022

[2] O. Tacar, P. Sriamornsak, C.R. Dass, Doxorubicin: an update on anticancer molecular action, toxicity and novel drug delivery systems, J Pharm Pharmacol, 65 (2013) 157-170.

DOI: https://doi.org/10.1111/j.2042-7158.2012.01567.x

[3] H. Maeda, G.Y. Bharate, J. Daruwalla, Polymeric drugs for efficient tumor-targeted drug delivery based on EPR-effect, Eur J Pharm Biopharm. 71 (2009) 409-419.

DOI: https://doi.org/10.1016/j.ejpb.2008.11.010

[4] L.E. Gerweck, K. Seetharaman, Cellular pH Gradient in Tumor versus Normal Tissue: Potential Exploitation for the Treatment of Cancer, Cancer Res. 56 (1996) 1194-1198.

[5] J. Carvalho, C. Gonçalves, A.M. Gil, F.M. Gama, Production and characterization of a new dextrin based hydrogel, Eur Polym J. 43 (2007) 3050-3059.

DOI: https://doi.org/10.1016/j.eurpolymj.2007.02.046

[6] S. Manchun, C.R. Dass, P. Sriamornsak, Designing nanoemulsion templates for fabrication of dextrin nanoparticles via emulsion cross-linking technique, Carbohydr Polym. 101 (2014) 650-655.

DOI: https://doi.org/10.1016/j.carbpol.2013.09.049

[7] D.N. Robinson, N.A. Peppas, Preparation and Characterization of pH-Responsive Poly(methacrylic acid-g-ethylene glycol) Nanospheres, Macromolecules. 35 (2002) 3668-3674.

DOI: https://doi.org/10.1021/ma011525u