Psychrophilic Carbonic Anhydrase Producing Bacteria for Screening and Optimizing the Enzyme Production
Carbonic anhydrase(CA)has a catalytic effect on calcium carbonate (CaCO3) deposition, and can also control the deposition of CaCO3. Screening strains that can produce carbonic anhydrase can solve the microbial CA from the natural environment in the role of CaCO3 deposition. Through the single and orthogonal test, the present paper focused on temperature, pH value, culture time and inoculation of 4 factors to optimally culture the psychrotrophic strains, which could produce CA by independent screening from Huanglong of cold calcified water, in order to get high yield of CA psychrophilic strains. The results showed that the optimal condition of the 15-33 strains producing enzyme was temperature 15 °C?, incubation time 12 h, pH 6.0, and inoculation 2.0 mL. The 18-10 strains, was temperature 15 °C?, incubation time 24 h, pH 6.5, and qinoculation 2.0 mL.
Seung-Bok Choi and Yun-Hae Kim
W. J. Zhang et al., "Psychrophilic Carbonic Anhydrase Producing Bacteria for Screening and Optimizing the Enzyme Production", Advanced Materials Research, Vols. 1092-1093, pp. 617-620, 2015