Research on Purifying Hypocrellin by Molecularly Imprinted Solid Phase Extraction (MISPE)
Separation and purification of hypocrellin A (HA) and hypocrellin B (HB) are conducted for extracted concentrated solution of hypocrellin through dry packing of molecularly imprinted polymers (MIPs) of hypocrellin A and B and preparative solid phase extraction column. The result shows that the optimum packing column combination for mixed adsorption is H5 (i.e. MIP of hypocrellin A and B are respectively 10.5g and 4.5g), and that the elution sequence is hypocrellin A elution first and then hypocrellin B elution next after the eluent is changed.
Seung-Bok Choi and Yun-Hae Kim
W. Y. Shi et al., "Research on Purifying Hypocrellin by Molecularly Imprinted Solid Phase Extraction (MISPE)", Advanced Materials Research, Vols. 1092-1093, pp. 621-624, 2015