Culture Medium Optimization for Photosynthetic Bacteria

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Photosynthetic bacteria (PSB) have various applications but the culture cost is very expensive. To find an efficient and economic culture medium the effects of yeast extract, C/N ratio, and trace elements on the growth of PSB were studied. The results showed that the optimal condition for PSB growth was: yeast extract of 100mg/l, C/N of 12:1, and trace elements (in mol/L): Mn2 + (0.009), Fe3+ (0.0025), Co2+ (0.0024), Cu2 + (0.0024), and Zn2 + (0.0033). Trace element lack could affect the growth of PSB. The order was Mn2 +> Fe3 +> Co2+> Cu2 +> Zn2 +. The improved medium was named HCH, and the optimum medium components were (in g/l) DL-malic acid: (4), MgSO4: (0.12), (NH4)2 SO4: (1), CaCl2: (0.075), KH2PO4: (0.5), K2HPO4: (0.3), Na2EDTA: (0.02), yeast extract : (0.1), trace elements 1ml (in mol/l ): Fe3+: (0.0025), Mn2+: (0.009), Zn2+: (0.0033), Co2+: (0.0024), pH:6.8. Comparing with the traditional RCVBN medium, in HCH medium yield of PSB increased 1.2 times and the cost decreased 19.8 times.

Info:

Periodical:

Advanced Materials Research (Volumes 113-116)

Edited by:

Zhenyu Du and X.B Sun

Pages:

1443-1446

DOI:

10.4028/www.scientific.net/AMR.113-116.1443

Citation:

W. Zhao et al., "Culture Medium Optimization for Photosynthetic Bacteria", Advanced Materials Research, Vols. 113-116, pp. 1443-1446, 2010

Online since:

June 2010

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$35.00

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