This study investigated the effects of different rates of phosphorus application on vertical transference of P. An aquatic brown paddy soil was filled in organic glass columns using for the leaching experiments. The results indicated that significant vertical transference was found using change of Olsen-P and CaCl2-P. The transference distance was no more than 10cm when P application rate between 200 and 800 kg P ha-1, and was more than 10cm as P application rate above 800 kg P ha-1. We obtained the threshold value of Olsen-P was 53.7 mg kg-1 by split-line model, and calculated that the threshold value of P fertilizer application rate was 382.1 kg P ha-1 by relationship of soil Olsen-P concentration and P application rate. The soil P transference increased, if P application rate was above 382.1 kg P ha-1. It would be helpful for controlling the P fertilizer amount by field to decrease P loss.