Properties of Phytase from Aspergillus oryzae093
Optimal condition, stability and Km of phytase which was from Aspergillus oryzae093 were studied. The optimal temperature and pH for sodium-phytate hydrolysis by Aspergillus oryzae093 phytase is 50°C and 5.5 respectively. Phytase has better heat stability as temperature below 45°C. The phytase activity remained 90% when pH is 5.0 to 6.0. Low concentration Fe2+ and Mg2+ can promote phytase activity, while Cu2+, Mn2+, Fe3+, Al3+ or high concentration Fe2+ and Mg2+ can inhibite the activity of phytase. The Km is about 4.8×10-5 mol•L-1 when sodium phytate as substrate. The enzyme properties of Aspergillus oryzae093 phytase make it could apply to food and feed to reduce the phosphorus exhausted to environment.
Zhenyu Du and X.B Sun
N. Zhang et al., "Properties of Phytase from Aspergillus oryzae093", Advanced Materials Research, Vols. 113-116, pp. 1833-1836, 2010