Properties of Phytase from Aspergillus oryzae093

Abstract:

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Optimal condition, stability and Km of phytase which was from Aspergillus oryzae093 were studied. The optimal temperature and pH for sodium-phytate hydrolysis by Aspergillus oryzae093 phytase is 50°C and 5.5 respectively. Phytase has better heat stability as temperature below 45°C. The phytase activity remained 90% when pH is 5.0 to 6.0. Low concentration Fe2+ and Mg2+ can promote phytase activity, while Cu2+, Mn2+, Fe3+, Al3+ or high concentration Fe2+ and Mg2+ can inhibite the activity of phytase. The Km is about 4.8×10-5 mol•L-1 when sodium phytate as substrate. The enzyme properties of Aspergillus oryzae093 phytase make it could apply to food and feed to reduce the phosphorus exhausted to environment.

Info:

Periodical:

Advanced Materials Research (Volumes 113-116)

Edited by:

Zhenyu Du and X.B Sun

Pages:

1833-1836

DOI:

10.4028/www.scientific.net/AMR.113-116.1833

Citation:

N. Zhang et al., "Properties of Phytase from Aspergillus oryzae093", Advanced Materials Research, Vols. 113-116, pp. 1833-1836, 2010

Online since:

June 2010

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Price:

$35.00

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