Study on Inter-Relationship of the Nutrients in Substrate and the Production of Demulsifying Compound by a Bacillus sp.

Abstract:

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This work investigated the effect of substrates on de-emulsification capability and the growth of a bacillus sp. (screened by our laboratory).The biomass and de-emulsification ratios(DR) of cultures grown in substrate with the different nutrients (glucose, liquid paraffin, NH4NO3 and yeast) were determined. In the orthogonal tests, the significant of de-emulsification capability in turn is glucose>liquid paraffin>yeast>NH4NO3. The glucose is the highest effect of nutrients on de-emulsification capability and growth. The de-emulsification ratios(DR) and the glucose utilization ratios increase simultaneously at log and lag growth phase. Measurements of the liquid paraffin residue indicates that 30.1% liquid paraffin in substrate is consumed and used in inducing the demulsifying compounds production rather than the increase of the biomass. The culture grown in substrate with 2%~5% liquid paraffin exhibits highest de-emulsification ratio(DR). The contents of yeast at 0.8mg/L~1.0mg/L is beneficial to the growth and demulsifying compound production.

Info:

Periodical:

Advanced Materials Research (Volumes 113-116)

Edited by:

Zhenyu Du and X.B Sun

Pages:

974-979

DOI:

10.4028/www.scientific.net/AMR.113-116.974

Citation:

X. Li et al., "Study on Inter-Relationship of the Nutrients in Substrate and the Production of Demulsifying Compound by a Bacillus sp.", Advanced Materials Research, Vols. 113-116, pp. 974-979, 2010

Online since:

June 2010

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$35.00

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