Biosynthesis of Gold Nanoparticles with Serratia marcescens Bacteria
This paper presents the biosynthesis of gold nanoparticles from the bacteria, Serratia marcescens. The intra-and extra-cellular synthesis of gold nanoparticles is shown to occur over a range of pH and incubation times in cell-free exracts and biomass of serratia marcescens that were reacted with 2.5mM Tetrachloroauric acid (HAuCl4). The formation of gold nanoparticles was identified initially via color changes from yellow auro-chloride to shades of red or purple in gold nanoparticle solutions. UV-Visible spectroscopy (UV-Vis), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray spectroscopy (EDS), Helium Ion Microscopy (HIM) and Dynamic Light Scattering (DLS) were also used to characterize gold nanoparticles produced within a range of pH conditions. The results show clearly that the production of gold nanoparticles from cell-free extracts require shorter times than the production of gold nanoparticles from the biomass.
Prof. Wole Soboyejo, Dr. Shola Odusunya, Dr. Zebaze Kana, Dr. Nicolas Anuku, Dr. Karen Malatesta and Dr. Mohammed Dauda
S.O. Dozie-Nwachukwu et al., "Biosynthesis of Gold Nanoparticles with Serratia marcescens Bacteria", Advanced Materials Research, Vol. 1132, pp. 19-35, 2016