Photocatalyst of perovskite type V-doped titanium-bearing blast furnace slag (VTBBFS) were prepared by high temperature solid-state method with different concentration of V for 2 hours at 800°C. The structure and composition of VTBBFS photocatalyst was characterized by XRD, UV-vis absorption spectra, and SEM images. It shows that VTBBFS photocatalyst has perovskite structure, and a kind of irregular and massive particles by calcined at 800°C. Its photo absorption performance was checked by UV-Vis spectrophotometer, its absorption spectrum has a obvious red shift. Use Aspergillus Niger to research the antibacterial activities of VTBBFS photocatalyst. The results show the antibacterial activities of VTBBFS photocatalyst has a good antibacterial performance with 5% diluent of VTBBFS15 under the UV light.