Atomic Force and Confocal Microscopic Studies of Collagen-Cell-Based Scaffolds for Vascular Tissue Engineering


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Collagen is the most used naturally occurring scaffold material. It’s a structural protein ubiquitous among mammalian. The ability of collagen type I to host different cell phenotype in vitro and its low antigenecity in vivo are well known. However, the principal drawback of collagenbased materials consists in their low mechanical properties. For vascular tissue engineering this represents a major limit, as the aim is to mimic the structure of a native vessel, which is known to be resistant and viscoelastic. Moreover, vascular cells are known to be susceptible in vivo to reorganize the matrix in which they proliferate. Therefore, the aim of this project is to study the micro structural organization of collagen-based scaffolds, and to assess the interactions between collagen and smooth muscle cells during regeneration. This knowledge will then allow the development of appropriate strategies to tailor the microstructure of the scaffold and its properties. Smooth muscle cells (SMCs) were selected to study the interactions between cells and matrix during the proliferation. Atomic Force Microscopy (AFM) in dry state in tapping mode and Confocal Laser Scanning Microscopy (CLSM) in reflection mode were used to investigate the microstructure of the scaffold. For the former technique cells were seeded on top of the collagen gel after jellification, while for the latter, cells were embedded into the collagen gel and stained with Rhodamine. The contact points between matrix and cells were investigated, as well as the capacity of vascular cells to induce a structural reorganization of collagen fibrils in the scaffold.



Advanced Materials Research (Volumes 15-17)

Edited by:

T. Chandra, K. Tsuzaki, M. Militzer and C. Ravindran




L. Amadori et al., "Atomic Force and Confocal Microscopic Studies of Collagen-Cell-Based Scaffolds for Vascular Tissue Engineering", Advanced Materials Research, Vols. 15-17, pp. 83-88, 2007

Online since:

February 2006




[1] R. Ross, Nature, Vol. 362 (1993), p.801.

[2] M. J. Plank, D. J. Wall and T. David, Prog Biophys Mol Biol, (2005).

[3] G. C. Lantz, S. F. Badylak, M. C. Hiles, A. C. Coffey, L. A. Geddes, K. Kokini, G. E. Sandusky and R. J. Morff, J Invest Surg, Vol. 6 (1993), p.297.

[4] Y. G. Wilson, M. G. Wyatt, I. C. Currie, R. N. Baird and P. M. Lamont, Eur J Vasc Endovasc Surg, Vol. 10 (1995), p.220.

[5] C. H. Lee, A. Singla and Y. Lee, Int J Pharm, Vol. 221 (2001), p.1.

[6] T. Hayashi and K. Mizuno, Creighton TE (ed) Encyclopedia of Molecular Biology, John Wiley & Sons, (1999).

[7] T. J. Wess, Adv Protein Chem, Vol. 70 (2005), p.341.

[8] P. L. Jones, F. S. Jones, B. Zhou and M. Rabinovitch, J Cell Sci, Vol. 112 ( Pt 4) (1999), p.435.

[9] M. Raspanti, T. Congiu and S. Guizzardi, Matrix Biol, Vol. 20 (2001), p.601.

[10] M. Venturoni, T. Gutsmann, G. E. Fantner, J. H. Kindt and P. K. Hansma, Biochem Biophys Res Commun, Vol. 303 (2003), p.508.

[11] M. Jastrzebska, B. Barwinski, I. Mroz, A. Turek, J. Zalewska-Rejdak and B. Cwalina, Eur Phys J E Soft Matter, (2005).

[12] F. Boccafoschi, J. Habermehl, N. Rajan, M. Cannas and D. Mantovani, Submitted to Biomaterials, (2006).

[13] F. Boccafoschi, J. Habermehl, S. Vesentini and D. Mantovani, Biomaterials, Vol. 26 (2005), p.7410.

[14] J. Habermehl, J. Skopinska, F. Boccafoschi, A. Sionkowska, H. Kaczmarek, G. Laroche and D. Mantovani, Macromol Bioscience, Vol. 5 (2005), p.821.


[15] J. A. Petruska and A. J. Hodge, Proc Natl Acad Sci U S A, Vol. 51 (1964), p.871.

[16] T. J. Wess, A. P. Hammersley, L. Wess and A. Miller, J Struct Biol, Vol. 122 (1998), p.92.

[17] V. Ottani, M. Raspanti and A. Ruggeri, Micron, Vol. 32 (2001), p.251.

[18] K. E. Kadler, D. F. Holmes, J. A. Trotter and J. A. Chapman, Biochem J, Vol. 316 ( Pt 1) (1996), p.1.

[19] D. E. Birk and R. L. Trelstad, J Cell Biol, Vol. 103 (1986), p.231.

[20] T. Gutsmann, G. E. Fantner, M. Venturoni, A. Ekani-Nkodo, J. B. Thompson, J. H. Kindt, D. E. Morse, D. K. Fygenson and P. K. Hansma, Biophys J, Vol. 84 (2003), p.2593.


[21] J. P. Stegemann, H. Hong and R. M. Nerem, J Appl Physiol, Vol. 98 (2005), p.2321.

[22] C. M. Shanahan and P. L. Weissberg, Arterioscler Thromb Vasc Biol, Vol. 18 (1998), p.333.

[23] K. Bilodeau, F. Couet, F. Boccafoschi and D. Mantovani, Artif Organs, Vol. 29 (2005), p.906.