Nine push-off specimens were designed, constructed, and tested. Test variables included reinforcement ratio of shear reinforcement, interface preparation, loading method and eccentricity of applied load. The failure pattern, hysteretic characteristic and degradation characteristic of capacity and stiffness of the specimens were analyzed. The effects of moment and cyclic reversed loading on interface shear capacity were studied. The results indicated that applied moment less than or equal to the flexural ultimate moment of shear plane has little influence on the interface shear capacity, and the effect of cyclic reversed loading on the interface shear capacity of new and existing concrete is bigger. The test value and the calculated value of current Code and Specification of China were compared. Results showed that the current JGJ 1-91 interface shear capacity provisions gave a very conservative estimate for interface shear capacity, while for the new and existing concrete interfaces under the action of cyclic reversed loading, the calculated value according to GB 50010-2002 tended to be unsafe.