As an important seismic analysis method, Pushover is widely used in high-rise buildings, while there is still lack of investigation on applicability of Pushover analysis on diagonal grid structural system. Two structures with height 144 and 288 meters are respectively built, and then Pushover analysis and Incremental dynamic analysis are conducted. Results calculated by two different methods are compared, including top displacement vs. base shear curve, inter-story drift vs. inter-story shear curve, distribution of inter-story drift angle along the building height and plastic developing sequence of structural weak positions. Meanwhile, influence of three lateral load patterns (uniform pattern，inverted triangle pattern and SRSS pattern) on the results is investigated. Analysis results demonstrate that Pushover analysis can in some extent reflect seismic performance of structures and SRSS load pattern can better capture global and local information of structures compared with other two patterns.