Dipicolinic Acid Contents Used for Estimating the Number of Spores in Raw Milk


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A new rapid detection the number of spores method had been developed in raw milk through analyzing dipicolinic acid. The method was based on the concentration of DPA in raw milk which was converted into endospore numbers using endospore forming pure cultures as standards. To increase the detection limit the extraction of DPA from endospores in raw milk was optimized. The results showed adding 0.5 mL of the 0.1 mol L-1 DTT solutions into centrifuged raw milk (4 mL containing spores) and incubating at 25°C for 30 min, then adding 2 mol L-1 HCl at boiling water for 30 min, the concentration of DPA was highest. The relationship between the absorbency (expressed in DPA) and the counts of spores was established. And the regression equation was obtained that is y = 0.1087 x - 0.3182, R2 = 0.7842. Thus, the methods of detection spores were ascertained in skim milk. The detection limits were 1.46×103 CFU mL-1.



Advanced Materials Research (Volumes 183-185)

Edited by:

Yanguo Shi and Jinlong Zuo




X. Han et al., "Dipicolinic Acid Contents Used for Estimating the Number of Spores in Raw Milk", Advanced Materials Research, Vols. 183-185, pp. 1467-1471, 2011

Online since:

January 2011




[1] P.T. Yung, H.S. Shafaat, S.A. Connon et. al: FEMS Microbiol Ecol. Vol. 59 (2007), p.300.

[2] J. Fichtel, H, Sass and J. Rullkotter: Food Control Vol. 19 (2008), p.1006.

[3] T.M. James, G. Atthur, M. L. Lorna: J Society Dairy Technology Vol. 47(4) (1994), p.111.

[4] L. Marilyn, Bach and C. Gilvarg: J Biol Chem. Vol. 241 (1966), p.4563.

[5] M.W. Tabor, J. Macgee, and J.W. Holland: Appl Environ Microb, Jan. (1976), p.25.

[6] C.H. White, R.R. Chang, J.H. Martin et. al: J Dairy Sci Vol. 57(11) (1974), p.1309.

[7] L. Wang, Y.M. Lin. Bioresource Technology. Vol. 98 (2007), p.3164.

[8] J. Fichtel, J. Koster, J. Rullkotter and H. Sass. FEMS Microbiol Ecol, Vol. 61 (2007), p.522.

[9] X. Han, L.W. Zhang, C.M. Fu. Chinese J Anal Chem, Vol. 35 (2007), p.1665.

[10] C.M. Cook, R.M. Saudeman: Aust J Dairy Technol, Vol. 55 (2000), P. 119.

[11] S. Furukawa, M. Shimoda and I. Hayakawa: J Appl Microbiol. Vol. 94 (2003), p.836.

[12] B. Setlow, X.A. Loshon, P.C. Genest, et. al.: J appl microbiol. Vol. 92, (2002), p.362.

[13] S. Furukawa, M. Shimoda, I. Hayakawa: J Appl Microbiol. Vol. 94. (2003), p.836.

[14] A. Rueckert, R.S. Ronimus, H.W. Morgan. J Microbiol Methlds. Vol. 60 (2005), p.155.

[15] C.H. White, R.R. Chang, J.H. Martin et. al: J Dairy Sci. Vol. 57 (1974), p.1309.

[16] Venkata R.V., Peter Setlow. FEMS microbiology letters Vol. 239 (2004), p.71.

[17] P.S. Fernandez, M.J. Oclo and A. Letters in Applied Microbiology Vol. 19 (1994), p.118.

[18] Q.M. LI, B.X. Yin, R.X. Rui. China dairy industry. Vol. 33(10) (2005), p.17.

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