Spectroscopic Studies on the Interaction between 2-Chlorophenol and Catalase

Abstract:

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The interaction characteristics of 2-chlorophenol (2-CP) with catalase (CAT) were investigated by employing fluorescence spectroscopy and UV-Vis absorption spectroscopy. The intrinsic fluorescence of CAT was quenched distinctly by 2-CP. The quenching mechanism of fluorescence of CAT by 2-CP was observed to be a static quenching procedure. The thermodynamic parameters indicated that the binding reaction was spontaneous and the hydrophobic force played the major role in stabilizing the 2-CP-CAT complex. The binding constant was 1.18×10-4 L/mol. The binding distance r and critical distance R0 was 1.90 nm and 1.64 nm, respectively.

Info:

Periodical:

Advanced Materials Research (Volumes 183-185)

Edited by:

Yanguo Shi and Jinlong Zuo

Pages:

206-210

DOI:

10.4028/www.scientific.net/AMR.183-185.206

Citation:

Q. Y. Liu "Spectroscopic Studies on the Interaction between 2-Chlorophenol and Catalase", Advanced Materials Research, Vols. 183-185, pp. 206-210, 2011

Online since:

January 2011

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$35.00

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