Immobilization of Lactase on Dialdehyde Oxycellulose-Coated Magnetite Nanoparticles
Dialdehyde oxycellulose-coated magnetite nanoparticles were prepared as carrier to immobilize the lactase, the preparation conditions and enzymatic properties of the immobilized enzyme were studied. The results showed that the maximum activity of immobilized enzyme is 0.424U/g when immobilized time is 4h, immobilized pH is 6.5, [enzyme]/[carrier] =1:15(g:g). Compared with free enzyme, the heat stability and basic resistance of immobilized lactase were increased. After reuse five times, the relative enzyme activity of immobilized enzyme was 63%.
Yanguo Shi and Jinlong Zuo
Q. Q. Guo et al., "Immobilization of Lactase on Dialdehyde Oxycellulose-Coated Magnetite Nanoparticles", Advanced Materials Research, Vols. 183-185, pp. 2063-2067, 2011