Immobilization of Lactase on Dialdehyde Oxycellulose-Coated Magnetite Nanoparticles

Abstract:

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Dialdehyde oxycellulose-coated magnetite nanoparticles were prepared as carrier to immobilize the lactase, the preparation conditions and enzymatic properties of the immobilized enzyme were studied. The results showed that the maximum activity of immobilized enzyme is 0.424U/g when immobilized time is 4h, immobilized pH is 6.5, [enzyme]/[carrier] =1:15(g:g). Compared with free enzyme, the heat stability and basic resistance of immobilized lactase were increased. After reuse five times, the relative enzyme activity of immobilized enzyme was 63%.

Info:

Periodical:

Advanced Materials Research (Volumes 183-185)

Edited by:

Yanguo Shi and Jinlong Zuo

Pages:

2063-2067

DOI:

10.4028/www.scientific.net/AMR.183-185.2063

Citation:

Q. Q. Guo et al., "Immobilization of Lactase on Dialdehyde Oxycellulose-Coated Magnetite Nanoparticles", Advanced Materials Research, Vols. 183-185, pp. 2063-2067, 2011

Online since:

January 2011

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Price:

$35.00

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