Inactivation of Escherichia coli in Orange Juice Using Pulsed Light(PL): Effect of Process Parameters and Physical Property

Ma, Feng Ming; Li, Pu; Li, Wen Zhu; Dong, Yu; Chen, Yu Lei

doi:10.4028/www.scientific.net/AMR.183-185.795

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Inactivation of Escherichia coli in Orange Juice Using Pulsed Light(PL): Effect of Process Parameters and Physical Property

Abstract:

This work studies the effect of imput voltage, number of pulsed,juice thickness, total soluble solids and suspended pulp on the inactivation of E. coli inoculated in an orange juice. The levels of microbial reduction was a direct correlation with the imput voltage and number of pulsed,a negative correlation with the juice thickness and the suspended pulp. In orange juice E. coli decreased by 2.3 log and 1.6 log after number of pulsed of 256 or 64,imput voltage of 4kV,respectively. These results indicate that this PL device could be used to improve microbial safety of orange juice.

Info:

Periodical:

Advanced Materials Research (Volumes 183-185)

Main Theme:

Environmental Biotechnology and Materials Engineering

Edited by:

Yanguo Shi and Jinlong Zuo

Pages:

795-799

DOI:

https://doi.org/10.4028/www.scientific.net/AMR.183-185.795

Citation:

F. M. Ma et al., "Inactivation of Escherichia coli in Orange Juice Using Pulsed Light(PL): Effect of Process Parameters and Physical Property", Advanced Materials Research, Vols. 183-185, pp. 795-799, 2011

Online since:

January 2011

Authors:

Feng Ming Ma, Pu Li, Wen Zhu Li, Yu Dong, Yu Lei Chen

Keywords:

Escherichia coli, Inactivation, Orange Juice, Pulsed Light, UV

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Paper TitlePages

Inactivation of Escherichia Coli on Titanium Dioxide Photocatalysis Nanoparticles

Authors: Jing Wang, Zhi Jiang Ji, Zhong He Shui, Xiao Yan Wang, Nan Ding, Hai Jian Li

Abstract: The effects of P25 TiO2 made by DEGUSSA and one kind nano-titanium dioxide made by a certain company in China (Expressed by Tc) on Escherichia coli were tested by the minimal inhibitory concentration (MIC) method, and the status dispersed of two kinds nano-TiO2 and the micro-phenomenon of the effect on Escherichia coli were observed by scanning electron microscope (SEM). The hydroxyl radical (•OH) signal intensity in liquid produced by nano-TiO2 was tested by electron spinning resonance (ESR). It was found that the MIC of the P25 and Tc were 500 mg/L and 5 g/L, respectively. The cells of E. coli which were attached by the nano-TiO2 particles were damaged strongly, and the membrane was dissolved and dispersed under SEM. The intensity of •OH signal produced by P25 was stronger than that of Tc tested by ESR. The size of E. coli was 0.4~0.7×1~3 µm, and the average diameter of nano-TiO2 was 20 nm. When the nano-TiO2 particles touch the E. coli, it would stick to the surface of the cell wall and destroy the cell membrane, lead to the canker of cell membrane and the outflow of cytoplasm. The difference of the MIC between the two kinds of nano-powders may be due to their dispersion degree and the particle diameter which influence the •OH production.

Dipicolinic Acid Contents Used for Estimating the Number of Spores in Raw Milk

Authors: Xue Han, Lan Wei Zhang, Feng Zhen, Hua Xi Yi, Ming Du, Li Li Zhang, Yan Hua Li, Wei Jun Wang

Abstract: A new rapid detection the number of spores method had been developed in raw milk through analyzing dipicolinic acid. The method was based on the concentration of DPA in raw milk which was converted into endospore numbers using endospore forming pure cultures as standards. To increase the detection limit the extraction of DPA from endospores in raw milk was optimized. The results showed adding 0.5 mL of the 0.1 mol L^-1 DTT solutions into centrifuged raw milk (4 mL containing spores) and incubating at 25°C for 30 min, then adding 2 mol L^-1 HCl at boiling water for 30 min, the concentration of DPA was highest. The relationship between the absorbency (expressed in DPA) and the counts of spores was established. And the regression equation was obtained that is y = 0.1087 x － 0.3182, R2 = 0.7842. Thus, the methods of detection spores were ascertained in skim milk. The detection limits were 1.46×103 CFU mL^-1.

1467

The Study of Pulsed White Light to Inactivation of Staphylococcus aureus in Drinking Water

Authors: Xing Bin Sun, Ming Ming Jiang, Feng Ming Ma, Meng Lin

Abstract: The effects of pulsed white light, input voltage, biological load, pH and turbidity on inactivation of Staphylococcus aureus in drinking water were studied. The results showed that the effect of pulsed white light on the inactivation of Staphylococcus aureus in drinking water was very significant. The concentration of S. aureus reduced to 1.0×101cfu/ml as the pulsed white light flashed for 8 times when the input voltage was 3000v. The inactivation increased with the plus of input voltage, and decreased with the added of biological load. The inactivation kept all most the same when pH is 5.0~9.0, and the effect of turbidity was not obvious when the turbidity was 5~25.

748

Electrochemical Drinking Water Disinfection with TiO_2-x Ceramic Electrodes at Flow Conditions

Authors: Viktorija Denisova, Linda Mezule, Tālis Juhna, Jurijs Ozolins

Abstract: Electrochemical disinfection device EDI-001 equipped with titanium oxide (TiO_2-x) ceramic electrodes was tested as a system for direct treatment of drinking water at flow conditions. Laboratory experiments were conducted to estimate the disinfection efficiency and main system parameters. Escherichia coli was selected as the model microorganism. The results have shown that contaminated tap water was completely disinfected within 15 minutes by using EDI-001.

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