16 strains of microorganisms were screened from garden soils which contain large amounts of insoluble phosphate rock, respectively, named HPP1-HPP16. HPP16 was selected to be further studied by reason of its high-performance phosphate-solubilizing ability. After a series of experiments about physiological-biochemical identification and 16SrDNA molecular biological identification, HPP16 had been identified as one strain of Enterobacter asburiae. After cultivation of HPP16 in liquid inorganic phosphorus medium, its activity achieved to an accumulations of soluble phosphate up to 1212.32 mg/L. The results showed that HPP16 has a great potential and a good prospect in crop farming.