A research focus on semi-solid metal processing is the preparation of semi-solid slurry with non-dendritic microstructure. During the past several decades, people tended to obtain the non-dendritic structure by stirring melt of alloy which downs to the semi-solid temperature range, such as mechanical stirring and electromagnetic stirring; In recent years, with the technological innovation of semi-solid slurry preparation turned to be more convenient and efficient, most of these processes are based on the control of nucleation and growth process of primary phase during solidification, such as NRC, SSR, SLC, SEED, and CRP. In this paper, a novel process, named as “Self-Inoculation Method (SIM)”, has been proposed for semi-solid slurry preparation. The process involves self-inoculants addition to the melt, and then pouring the melt to mould through a multi-stream mixed cooling channel. The melt was avoided chemical pollution due to the particles of self-inoculants from the same composition as the melt. The semi-solid billets of AM60 alloy with non-dendritic structure were prepared by SIM. The effects of process parameters on the microstructure and the mechanism on refinement of alloy were investigated. The results indicate that pouring temperature, addition amount of self-inoculants and slope angle of the cooling channel are key factors for SIM process. The optimized parameters for the billet preparation of AM60 alloy are obtained: pouring temperature is at 680°C~700°C；addition of self-inoculants are between 5%~7% (mass fraction)；slope angle of the cooling channel is at 30°~45°. The heterogeneous nucleation was enhanced as the addition of self-inoculants; the formation of chill crystal and the fragmentation of dendrites because of cooling and shearing of the cooling channel, resulting in the increase of grains density and a small grain size.