Integrated System to Biological Solubilization and Precipitation of Heavy Metals


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The work consists on the study of a sulphur–oxidizing bacteria (At. thiooxidans) immobilisation over polyurethane foam and the integration of two continuous processes: the solubilization of heavy metals by acidic medium generated by sulphur-oxidizing bacteria and the subsequent precipitation of metals as sulphides with H2S biologically generated by sulphate-reducing bacteria (Desulfovibrio sp). At. thiooxidans was satisfactory immobilised over polyurethane foam and added to a column reactor. Acidic medium generated was added to a column with 50 g of an artificial contaminated sand (85 mg Cr(III), 20 mg Ni(II), 200 mg Zn(II)). The effluent of this step was collected in a reservoir tank, in which H2S from sulphate-reducing reactor was included to carry out the precipitation of metals. After 2.4 l of acid medium was passed through the column, it was observed that 14.6% of Cr(III), 26.7% of Ni(II) and 90.5% of Zn(II) were solubilized. The leachate was treated with 2.2 l of reducing medium, and 2.2% Cr(III),54% Ni(II) and 28% Zn(II) were precipitated.



Advanced Materials Research (Volumes 20-21)

Edited by:

Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher






G. Cabrera et al., "Integrated System to Biological Solubilization and Precipitation of Heavy Metals", Advanced Materials Research, Vols. 20-21, pp. 279-282, 2007

Online since:

July 2007




[1] A. Ballester, F. González and M.L. Blázquez: Hydrometallurgy Vol. 29 (1992) pp.145-160.

[2] A.I. Zouboulis, K.A. Matis and I.C. Handcock IC.: Separation and Purification Methods, Vol. 26 (1997), pp.255-295.

[3] J.R. Lloyd, A.N. Mabbett, D.R. Williams, L.E. Macaskie: Hydrometallurgy, Vol. 59 (2001), pp.327-337.

[4] O. Tuovinen; in: Microbial Mineral Recovery. McGraw-Hill, New York. (1990).

[5] S. Foucher, F. Battaglia-Brunet, I. Ignatiadis, D. Morin, Chem. Eng. Sci. Vol. 56 (2001), pp.1639-1645.

[6] C. White, G.M. Gadd, In: Extremofiles: Microbial Life in Extreme Environments, Wiley-Liss, Inc., New York (1998).

[7] S.D. Kim, J.J. Kilbane, D.K. Cha: Environ. Eng. Sci. Vol. 16 (1999), pp.139-145.

[8] C. White, A.K. Sharman, G.M. Gadd: Nat. Biotechnol. Vol. 16 (1998), pp.572-575.

[9] M.P. Silverman and D.G. Lundgren: J. Bacteriol. Vol. 77(1959), pp.642-647.

[10] G. Cabrera, R. Pérez, J.M. Gómez, A. Ábalo and D. Cantero: Journal of Hazardous Materials, Vol. A135 (2006), pp.40-46.

[11] I. de Ory, G. Cabrera, M. Ramirez and A. Blandino: in : Methods in Immobilization of Enzymes and Cells edited by Humana Press. Editor: J.M. Guisan (2º ed). Chapter 31 (2005) pp.357-365.

[12] L.S. Clesceri, A.E. Greenberg, A.E. Eaton; in: Plasma Emission Spectroscopy. Standard Methods for the Examination of Water and Wastewater. APHA, AWWA, WEF, 20th ed. Washington DC (1998).

[13] J.M. Gómez, I. Caro, D. Cantero: J. Biotechnol. Vol. 48 (1996).

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